ArticleMolecular and cellular biology2004
Role of pre-rRNA base pairing and 80S complex formation in subnucleolar localization of the U3 snoRNP.
Article in Molecular and cellular biology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 42 citations in OpenAlex.
- Current research on viral proteins that interact with fibrillarin.Molecular biology reports · 2023Review
- The human box C/D snoRNA U3 is a miRNA source and miR-U3 regulates expression of sortin nexin 27.Nucleic acids research · 2020Article
- Impaired chondrocyte U3 snoRNA expression in osteoarthritis impacts the chondrocyte protein translation apparatus.Scientific reports · 2020Article
- Fibrillarin Ribonuclease Activity is Dependent on the GAR Domain and Modulated by Phospholipids.Cells · 2020Article
- C/D box snoRNAs in viral infections: RNA viruses use old dogs for new tricks.Non-coding RNA research · 2019Review
- Interactions and activities of factors involved in the late stages of human 18S rRNA maturation.RNA biology · 2019Article
- Small Nucleolar RNAs: Insight Into Their Function in Cancer.Frontiers in oncology · 2019Review
- Implication of the box C/D snoRNP assembly factor Rsa1p in U3 snoRNP assembly.Nucleic acids research · 2017Article
- C/D-box snoRNAs form methylating and non-methylating ribonucleoprotein complexes: Old dogs show new tricks.BioEssays : news and reviews in molecular, cellular and developmental biology · 2017Review
- Robust statistical modeling improves sensitivity of high-throughput RNA structure probing experiments.Nature methods · 2017Article
- The NF45/NF90 Heterodimer Contributes to the Biogenesis of 60S Ribosomal Subunits and Influences Nucleolar Morphology.Molecular and cellular biology · 2015Article
- Structural and functional analysis of the U3 snoRNA binding protein Rrp9.RNA (New York, N.Y.) · 2013Article
- NOL11, implicated in the pathogenesis of North American Indian childhood cirrhosis, is required for pre-rRNA transcription and processing.PLoS genetics · 2012Article
- Transcriptional repressor NIR functions in the ribosome RNA processing of both 40S and 60S subunits.PloS one · 2012Article
- A second base pair interaction between U3 small nucleolar RNA and the 5'-ETS region is required for early cleavage of the yeast pre-ribosomal RNA.Nucleic acids research · 2011Article
- Elucidation of the assembly events required for the recruitment of Utp20, Imp4 and Bms1 onto nascent pre-ribosomes.Nucleic acids research · 2011Article
- Splicing factor 2-associated protein p32 participates in ribosome biogenesis by regulating the binding of Nop52 and fibrillarin to preribosome particles.Molecular & cellular proteomics : MCP · 2011Article
- The initial U3 snoRNA:pre-rRNA base pairing interaction required for pre-18S rRNA folding revealed by in vivo chemical probing.Nucleic acids research · 2011Article
- A weak C' box renders U3 snoRNA levels dependent on hU3-55K binding.Molecular and cellular biology · 2011Article
- Sequence and generation of mature ribosomal RNA transcripts in Dictyostelium discoideum.The Journal of biological chemistry · 2011Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In the nucleolus the U3 snoRNA is recruited to the 80S pre-rRNA processing complex in the dense fibrillar component (DFC). The U3 snoRNA is found throughout the nucleolus and has been proposed to move with the preribosomes to the granular component (GC). In contrast, the localization of other RNAs, such as the U8 snoRNA, is restricted to the DFC. Here we show that the incorporation of the U3 snoRNA into the 80S processing complex is not dependent on pre-rRNA base pairing sequences but requires the B/C motif, a U3-specific protein-binding element. We also show that the binding of Mpp10 to the 80S U3 complex is dependent on sequences within the U3 snoRNA that base pair with the pre-rRNA adjacent to the initial cleavage site. Furthermore, mutations that inhibit 80S complex formation and/or the association of Mpp10 result in retention of the U3 snoRNA in the DFC. From this we propose that the GC localization of the U3 snoRNA is a direct result of its active involvement in the initial steps of ribosome biogenesis.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.