ArticleMolecular and cellular biology2004
Characterization of karyopherin cargoes reveals unique mechanisms of Kap121p-mediated nuclear import.
Article in Molecular and cellular biology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed.
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- Chaperoning RPA during DNA metabolism.Current genetics · 2019Review
- Engineering metabolite-responsive transcriptional factors to sense small molecules in eukaryotes: current state and perspectives.Microbial cell factories · 2019Review
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- Alternative Use of DNA Binding Domains by the Neurospora White Collar Complex Dictates Circadian Regulation and Light Responses.Molecular and cellular biology · 2015Article
- NPC mimics: probing the mechanism of nucleocytoplasmic transport.Methods in cell biology · 2014Article
- Nucleocytoplasmic transport: a role for nonspecific competition in karyopherin-nucleoporin interactions.Molecular & cellular proteomics : MCP · 2012Article
- Identification of a nuclear localization sequence in β-arrestin-1 and its functional implications.The Journal of biological chemistry · 2012Article
- The yeast nuclear pore complex and transport through it.Genetics · 2012Article
- LAS1L interacts with the mammalian Rix1 complex to regulate ribosome biogenesis.Molecular biology of the cell · 2012Article
- Nuclear import by karyopherin-βs: recognition and inhibition.Biochimica et biophysica acta · 2011Review
- Ribosome synthesis-unrelated functions of the preribosomal factor Rrp12 in cell cycle progression and the DNA damage response.Molecular and cellular biology · 2011Article
- Article
- Efficient nuclear transport of structurally disturbed cargo: mutations in a cargo protein switch its cognate karyopherin.PloS one · 2011Article
- Mechanisms and signals for the nuclear import of proteins.Current genomics · 2009Article
- The eIF3 interactome reveals the translasome, a supercomplex linking protein synthesis and degradation machineries.Molecular cell · 2009Article
- Kap104p imports the PY-NLS-containing transcription factor Tfg2p into the nucleus.The Journal of biological chemistry · 2009Article
- Article
- A PY-NLS nuclear targeting signal is required for nuclear localization and function of the Saccharomyces cerevisiae mRNA-binding protein Hrp1.The Journal of biological chemistry · 2008Article
- The Hsp40 chaperone Jjj1 is required for the nucleo-cytoplasmic recycling of preribosomal factors in Saccharomyces cerevisiae.RNA (New York, N.Y.) · 2007Article
Corrections and comments
- Erratum issued
Authors and funding
7 authors.
Funding
Abstract
In yeast there are at least 14 members of the beta-karyopherin protein family that govern the movement of a diverse set of cargoes between the nucleus and cytoplasm. Knowledge of the cargoes carried by each karyopherin and insight into the mechanisms of transport are fundamental to understanding constitutive and regulated transport and elucidating how they impact normal cellular functions. Here, we have focused on the identification of nuclear import cargoes for the essential yeast beta-karyopherin, Kap121p. Using an overlay blot assay and coimmunopurification studies, we have identified 30 putative Kap121p cargoes. Among these were Nop1p and Sof1p, two essential trans-acting protein factors required at the early stages of ribosome biogenesis. Characterization of the Kap121p-Nop1p and Kap121p-Sof1p interactions demonstrated that, in addition to lysine-rich nuclear localization signals (NLSs), Kap121p recognizes a unique class of signals distinguished by the abundance of arginine and glycine residues and consequently termed rg-NLSs. Kap104p is also known to recognize rg-NLSs, and here we show that it compensates for the loss of Kap121p function. Sof1p is also transported by Kap121p; however, its import can be mediated by a piggyback mechanism with Nop1p bridging the interaction between Sof1p and Kap121p. Together, our data elucidate additional levels of complexity in these nuclear transport pathways.
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