ArticleJournal of virology2004
A riboswitch regulates RNA dimerization and packaging in human immunodeficiency virus type 1 virions.
Article in Journal of virology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 70 papers.
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Who cites it
70 citing papers in PubMed, 104 citations in OpenAlex.
- Distinct Gag interaction properties of HIV-1 RNA 5' leader conformers reveal a mechanism for dimeric genome selection.RNA (New York, N.Y.) · 2023Article
- Editorial: RNA machines.Frontiers in genetics · 2023Article
- Human Retrovirus Genomic RNA Packaging.Viruses · 2022Review
- Short- and long-range interactions in the HIV-1 5' UTR regulate genome dimerization and packaging.Nature structural & molecular biology · 2022Article
- A novel role for gag as a cis-acting element regulating RNA structure, dimerization and packaging in HIV-1 lentiviral vectors.Nucleic acids research · 2022Article
- HibeRNAtion: HIV-1 RNA Metabolism and Viral Latency.Frontiers in cellular and infection microbiology · 2022Review
- Stability and conformation of the dimeric HIV-1 genomic RNA 5'UTR.Biophysical journal · 2021Article
- 5'-Cap sequestration is an essential determinant of HIV-1 genome packaging.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Review
- Development of a Novel Competitive qRT-PCR Assay to Measure Relative Lentiviral Packaging Efficiency.Molecular therapy. Methods & clinical development · 2020Article
- Unpaired Guanosines in the 5' Untranslated Region of HIV-1 RNA Act Synergistically To Mediate Genome Packaging.Journal of virology · 2020Article
- NMR Studies of Retroviral Genome Packaging.Viruses · 2020Review
- Overview of the Nucleic-Acid Binding Properties of the HIV-1 Nucleocapsid Protein in Its Different Maturation States.Viruses · 2020Review
- Identification of the initial nucleocapsid recognition element in the HIV-1 RNA packaging signal.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- HIV-1 spliced RNAs display transcription start site bias.RNA (New York, N.Y.) · 2020Article
- Structural basis for transcriptional start site control of HIV-1 RNA fate.Science (New York, N.Y.) · 2020Article
- Intrinsic conformational dynamics of the HIV-1 genomic RNA 5'UTR.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- RNA 3-dimensional structural motifs as a critical constraint of viroid RNA evolution.PLoS pathogens · 2018Article
- Article
- The C-terminal p6 domain of the HIV-1 Pr55RNA biology · 2018Article
10 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The genome of retroviruses, including human immunodeficiency virus type 1 (HIV-1), consists of two identical RNA strands that are packaged as noncovalently linked dimers. The core packaging and dimerization signals are located in the downstream part of the untranslated leader of HIV-1 RNA-the Psi and the dimerization initiation site (DIS) hairpins. The HIV-1 leader can adopt two alternative conformations that differ in the presentation of the DIS hairpin and consequently in their ability to dimerize in vitro. The branched multiple-hairpin (BMH) structure folds the poly(A) and DIS hairpins, but these domains are base paired in a long distance interaction (LDI) in the most stable LDI conformation. This LDI-BMH riboswitch regulates RNA dimerization in vitro. It was recently shown that the Psi hairpin structure is also presented differently in the LDI and BMH structures. Several detailed in vivo studies have indicated that sequences throughout the leader RNA contribute to RNA packaging, but how these diverse mutations affect the packaging mechanism is not known. We reasoned that these effects may be due to a change in the LDI-BMH equilibrium, and we therefore reanalyzed the structural effects of a large set of leader RNA mutations that were presented in three previous studies (J. L. Clever, D. Mirandar, Jr., and T. G. Parslow, J. Virol. 76:12381-12387, 2002; C. Helga-Maria, M. L. Hammarskjold, and D. Rekosh, J. Virol. 73:4127-4135, 1999; R. S. Russell, J. Hu, V. Beriault, A. J. Mouland, M. Laughrea, L. Kleiman, M. A. Wainberg, and C. Liang, J. Virol. 77:84-96, 2003). This analysis revealed a strict correlation between the status of the LDI-BMH equilibrium and RNA packaging. Furthermore, a correlation is apparent between RNA dimerization and RNA packaging, and these processes may be coordinated by the same LDI-BMH riboswitch mechanism.
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