ArticleJournal of virology2004
Forced selection of a human immunodeficiency virus type 1 variant that uses a non-self tRNA primer for reverse transcription: involvement of viral RNA sequences and the reverse transcriptase enzyme.
Article in Journal of virology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 32 citations in OpenAlex.
- Low-level alternative tRNA priming of reverse transcription of HIV-1 and SIV in vivo.Retrovirology · 2019Article
- Dynamic Interplay of RNA and Protein in the Human Immunodeficiency Virus-1 Reverse Transcription Initiation Complex.Journal of molecular biology · 2018Article
- HIV-1 Employs Multiple Mechanisms To Resist Cas9/Single Guide RNA Targeting the Viral Primer Binding Site.Journal of virology · 2018Article
- HIV-1 Subtypes and 5'LTR-Leader Sequence Variants Correlate with Seroconversion Status in Pumwani Sex Worker Cohort.Viruses · 2017Article
- RNase H sequence preferences influence antisense oligonucleotide efficiency.Nucleic acids research · 2017Article
- Heterogeneous structures formed by conserved RNA sequences within the HIV reverse transcription initiation site.RNA (New York, N.Y.) · 2016Article
- New insights into inhibition of human immunodeficiency virus type 1 replication through mutant tRNALys3.Retrovirology · 2013Article
- The Interaction between tRNA(Lys) 3 and the primer activation signal deciphered by NMR spectroscopy.PloS one · 2013Article
- HIV infection and HERV expression: a review.Retrovirology · 2012Review
- Initiation of HIV Reverse Transcription.Viruses · 2010Article
- tRNA isoacceptor preference prior to retrovirus Gag-Pol junction links primer selection and viral translation.Journal of virology · 2007Article
- Analysis of the replication of HIV-1 forced to use tRNAMet(i) supports a link between primer selection, translation and encapsidation.Retrovirology · 2007Article
- The availability of the primer activation signal (PAS) affects the efficiency of HIV-1 reverse transcription initiation.Nucleic acids research · 2007Article
- Second site escape of a T20-dependent HIV-1 variant by a single amino acid change in the CD4 binding region of the envelope glycoprotein.Retrovirology · 2006Article
- Complementation of human immunodeficiency virus type 1 replication by intracellular selection of Escherichia coli formula supplied in trans.Journal of virology · 2006Article
- HIV-1 designed to use different tRNAGln isoacceptors prefers to select tRNAThr for replication.Virology journal · 2006Article
- The interplay between virus infection and the cellular RNA interference machinery.FEBS letters · 2006Review
- Inhibition of human immunodeficiency virus type 1 replication with artificial transcription factors targeting the highly conserved primer-binding site.Journal of virology · 2006Article
- Hairpin-induced tRNA-mediated (HITME) recombination in HIV-1.Nucleic acids research · 2006Article
- Defective replication in human immunodeficiency virus type 1 when non-primers are used for reverse transcription.Journal of virology · 2005Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Human immunodeficiency virus type 1 uses the tRNA(3)(Lys) molecule as a selective primer for reverse transcription. This primer specificity is imposed by sequence complementarity between the tRNA primer and two motifs in the viral RNA genome: the primer-binding site (PBS) and the primer activation signal (PAS). In addition, there may be specific interactions between the tRNA primer and viral proteins, such as the reverse transcriptase (RT) enzyme. We constructed viruses with mutations in the PAS and PBS that were designed to employ the nonself primer tRNA(Pro) or tRNA(1,2)(Lys). These mutants exhibited a severe replication defect, indicating that additional adaptation of the mutant virus is required to accommodate the new tRNA primer. Multiple independent virus evolution experiments were performed to select for fast-replicating variants. Reversion to the wild-type PBS-lys3 sequence was the most frequent escape route. However, we identified one culture in which the virus gained replication capacity without reversion of the PBS. This revertant virus eventually optimized the PAS motif for interaction with the nonself primer. Interestingly, earlier evolution samples revealed a single amino acid change of an otherwise well-conserved residue in the RNase H domain of the RT enzyme, implicating this domain in selective primer usage. We demonstrate that both the PAS and RT mutations improve the replication capacity of the tRNA(1,2)(Lys)-using virus.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.