ArticleJournal of virology2004
Short interfering RNA-mediated inhibition of herpes simplex virus type 1 gene expression and function during infection of human keratinocytes.
Article in Journal of virology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
22 citing papers in PubMed, 53 citations in OpenAlex.
- A review on current status of antiviral siRNA.Reviews in medical virology · 2018Review
- Adenovirus-mediated shRNA interference against HSV-1 replication in vitro.Journal of neurovirology · 2016Article
- Decrease of murine cytomegalovirus-induced retinitis by intravenous delivery of immediate early protein-3-specific siRNA.Investigative ophthalmology & visual science · 2014Article
- Silencing herpes simplex virus type 1 capsid protein encoding genes by siRNA: a promising antiviral therapeutic approach.PloS one · 2014Article
- Analysis of virion-incorporated host proteins required for herpes simplex virus type 1 infection through a RNA interference screen.PloS one · 2013Article
- Small interfering RNA targeting for infected-cell polypeptide 4 inhibits herpes simplex virus type 1 replication in retinal pigment epithelial cells.Clinical & experimental ophthalmology · 2012Article
- Targeting herpetic keratitis by gene therapy.Journal of ophthalmology · 2012Article
- Article
- Enhanced inhibition of Avian leukosis virus subgroup J replication by multi-target miRNAs.Virology journal · 2011Article
- Antiviral RNAi: translating science towards therapeutic success.Pharmaceutical research · 2011Review
- Interleukin 29 enhances expression of Toll receptor 3 and mediates antiviral signals in human keratinocytes.Inflammation research : official journal of the European Histamine Research Society ... [et al.] · 2011Article
- The effect of murine cytomegalovirus IE-3 specific shRNA is dependent on intragenic target site due to multiple transcription initiation sites.Herpesviridae · 2011Article
- Optimizing siRNA delivery to the genital mucosa.Discovery medicine · 2011Review
- Effect of siRNAs on HSV-1 plaque formation and relative expression levels of RR mRNA.Virologica Sinica · 2011Article
- Inhibition of herpes simplex virus type 1 by small interfering RNA.Clinical and experimental dermatology · 2008Article
- RNA interference for antiviral therapy.The journal of gene medicine · 2006Review
- Inhibition of viruses by RNA interference.Virus genes · 2006Review
- RNA interference: its use as antiviral therapy.Handbook of experimental pharmacology · 2006Review
- New therapeutics targets in chronic viral cardiomyopathy.Ernst Schering Research Foundation workshop · 2006Review
- The human herpesvirus 6 G protein-coupled receptor homolog U51 positively regulates virus replication and enhances cell-cell fusion in vitro.Journal of virology · 2005Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
RNA interference (RNAi) is an antiviral mechanism that is activated when double-stranded RNA is cleaved into fragments, called short interfering RNA (siRNA), that prime an inducible gene silencing enzyme complex. We applied RNAi against a herpes simplex virus type 1 (HSV-1) gene, glycoprotein E, which mediates cell-to-cell spread and immune evasion. In an in vitro model of infection, human keratinocytes were transfected with siRNA specific for glycoprotein E and then infected with wild-type HSV-1. RNAi-mediated gene silencing reproduced the small plaque phenotype of a gE-deletion mutant virus. The specificity of gene targeting was demonstrated by flow cytometry and Northern blot analyses. Exogenous siRNA can suppress HSV-1 glycoprotein E expression and function during active infection in vitro through RNAi. This work establishes RNAi as a genetic tool for the study of HSV and provides a foundation for development of RNAi as a novel antiviral therapy.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.