ArticleJournal of virology2004
Vaccinia virus mutants with alanine substitutions in the conserved G5R gene fail to initiate morphogenesis at the nonpermissive temperature.
Article in Journal of virology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed, 30 citations in OpenAlex.
- Pathogens That Rewrite the Rules: Ascoviruses, Elegant Manipulators of Cell Death Pathways and Architects of the Extracellular Viral Paradigm.Pathogens (Basel, Switzerland) · 2025Review
- Temporal expression classes and functions of vaccinia virus and mpox (monkeypox) virus genes.mBio · 2025Review
- Identification of protective T-cell antigens for smallpox vaccines.Cytotherapy · 2020Article
- Protein Primary Structure of the Vaccinia Virion at Increased ResolutionJournal of virology · 2016Article
- Fine structure of the vaccinia virion determined by controlled degradation and immunolocalization.Virology · 2015Article
- Human antibody responses to the polyclonal Dryvax vaccine for smallpox prevention can be distinguished from responses to the monoclonal replacement vaccine ACAM2000.Clinical and vaccine immunology : CVI · 2014Article
- Vaccinia virus A6 is essential for virion membrane biogenesis and localization of virion membrane proteins to sites of virion assembly.Journal of virology · 2012Article
- Vaccinia virus L2 protein associates with the endoplasmic reticulum near the growing edge of crescent precursors of immature virions and stabilizes a subset of viral membrane proteins.Journal of virology · 2011Article
- Participation of vaccinia virus l2 protein in the formation of crescent membranes and immature virions.Journal of virology · 2011Article
- Lipid membranes in poxvirus replication.Viruses · 2010Article
- Poxvirus proteomics and virus-host protein interactions.Microbiology and molecular biology reviews : MMBR · 2009Review
- Predicted poxvirus FEN1-like nuclease required for homologous recombination, double-strand break repair and full-size genome formation.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Vaccinia virus H7 protein contributes to the formation of crescent membrane precursors of immature virions.Journal of virology · 2009Article
- Poxvirus DNA primase.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- Vaccinia virus A6L encodes a virion core protein required for formation of mature virion.Journal of virology · 2007Article
- Existence of an operative pathway from the endoplasmic reticulum to the immature poxvirus membrane.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- Genomic sequence of Spodoptera frugiperda Ascovirus 1a, an enveloped, double-stranded DNA insect virus that manipulates apoptosis for viral reproduction.Journal of virology · 2006Article
- Vaccinia virus proteome: identification of proteins in vaccinia virus intracellular mature virion particles.Journal of virology · 2006Article
- Vaccinia virus nonstructural protein encoded by the A11R gene is required for formation of the virion membrane.Journal of virology · 2005Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
The initial characterization of the product of the vaccinia virus G5R gene, which is conserved in all poxviruses sequenced to date, is described. The G5 protein was detected in the core fraction of purified virions, and transcription and translation of the G5R open reading frame occurred early in infection, independently of DNA replication. Attempts to delete the G5R gene and isolate a replication-competent virus were unsuccessful, suggesting that G5R encodes an essential function. We engineered vaccinia virus mutants with clusters of charged amino acids changed to alanines and determined that several were unable to replicate at 40 degrees C but grew well at 37 degrees C. At the nonpermissive temperature, viral gene expression and DNA replication and processing were unperturbed. However, tyrosine phosphorylation and proteolytic cleavage of the A17 membrane protein and proteolytic cleavage of core proteins were inhibited at 40 degrees C, suggesting an assembly defect. The cytoplasm of cells that had been infected at the nonpermissive temperature contained large granular areas devoid of cellular organelles or virus structures except for occasional short crescent-shaped membranes and electron-dense lacy structures. The temperature-sensitive phenotype of the G5R mutants closely resembled the phenotypes of vaccinia virus mutants carrying conditionally lethal F10R protein kinase and H5R mutations. F10, although required for phosphorylation of A17 and viral membrane formation, was synthesized by the G5R mutants under nonpermissive conditions. An intriguing possibility is that G5 participates in the formation of viral membranes, a poorly understood event in poxvirus assembly.
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