ArticleJournal of infection and chemotherapy : official journal of the Japan Society of Chemotherapy2004
Ultrasonic-enhanced gentamicin transport through colony biofilms of Pseudomonas aeruginosa and Escherichia coli.
Article in Journal of infection and chemotherapy : official journal of the Japan Society of Chemotherapy, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers, 1 of them a synthesis that pooled it.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
47 citing papers in PubMed, 1 synthesis or guideline pooled it, 116 citations in OpenAlex.
- Custom-Made Antibiotic Cement Nails in Orthopaedic Trauma: Review of Outcomes, New Approaches, and Perspectives.BioMed research international · 2015Pooled it
- The effect of 22.5 kHz low-frequency contact ultrasound debridement (LFCUD) on lower extremity wound healing for a vascular surgery population: A randomised controlled trial.International wound journal · 2018Trial
- Percutaneous extraction using sponge spicules for disease diagnosis and therapeutic drug monitoring.Scientific reports · 2025Article
- Effect of biofilm physical characteristics on their susceptibility to antibiotics: impacts of low-frequency ultrasound.NPJ biofilms and microbiomes · 2024Article
- Experiment to Demonstrate Pesticide-Induced Antimicrobial Resistance (AMR): An Emerging Health Threat.Cureus · 2024Article
- Measuring Niche-Associated Metabolic Activity in Planktonic and Biofilm Bacteria.Methods in molecular biology (Clifton, N.J.) · 2023Article
- The Combination of Low-Frequency Ultrasound and Antibiotics Improves the Killing of In VitroAntibiotics (Basel, Switzerland) · 2022Article
- Specific phenotypic, genomic, and fitness evolutionary trajectories toward streptomycin resistance induced by pesticide co-stressors in Escherichia coli.ISME communications · 2021Article
- Bovine serum promotes the formation and phenotype memory retention of persister cells in Escherichia coli liquid cultures.World journal of microbiology & biotechnology · 2021Article
- Potential of Carvacrol and Thymol in Reducing Biofilm Formation on Technical Surfaces.Molecules (Basel, Switzerland) · 2021Article
- Sonobactericide: An Emerging Treatment Strategy for Bacterial Infections.Ultrasound in medicine & biology · 2020Review
- Low-Frequency Ultrasound Enhances Bactericidal Activity of Antimicrobial Agents againstBioMed research international · 2020Article
- Review
- Quantitative analysis ofIranian journal of microbiology · 2018Article
- Bacterial biofilm formation on implantable devices and approaches to its treatment and prevention.Heliyon · 2018Review
- Laser-induced vapour nanobubbles improve drug diffusion and efficiency in bacterial biofilms.Nature communications · 2018Article
- Low-intensity pulsed ultrasound enhances antibiotic release of gentamicin-loaded, self-setting calcium phosphate cement.The Journal of international medical research · 2018Article
- Effect of Low-Frequency Pulsed Ultrasound on Drug Delivery, Antibacterial Efficacy, and Bone Cement Degradation in Vancomycin-Loaded Calcium Phosphate Cement.Medical science monitor : international medical journal of experimental and clinical research · 2018Article
- The synergistic bactericidal effect of vancomycin on UTMD treated biofilm involves damage to bacterial cells and enhancement of metabolic activities.Scientific reports · 2018Article
- Bacterial Memory of Persisters: Bacterial Persister Cells Can Retain Their Phenotype for Days or Weeks After Withdrawal From Colony-Biofilm Culture.Frontiers in microbiology · 2018Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
The hypothesis that ultrasound increases antibiotic transport through biofilms of Escherichia coli and Pseudomonas aeruginosa was investigated using colony biofilms. Biofilms grown on membrane filters were transferred to nutrient agar containing 50 microg/ml gentamicin. A smaller filter was placed on top of the biofilm and a blank concentration disk was situated atop the filter. Diffusion of antibiotic through the biofilms was allowed for 15, 30, or 45 min at 37 degrees C. Some of these biofilms were exposed to 70-kHz ultrasound and others were not. Each concentration disk was then placed on a nutrient agar plate spread with a lawn of E. coli. The resulting zone of inhibition was used to calculate the amount of gentamicin that was transported through the biofilm into the disk. The E. coli and P. aeruginosa biofilms grown for 13 and 24 h were exposed to two different ultrasonic power densities. Ultrasonication significantly increased the transport of gentamicin through the biofilm. Insonation of biofilms of E. coli for 45 min more than doubled the amount of gentamicin compared to their noninsonated counterparts. For P. aeruginosa biofilms, no detectable gentamicin penetrated the biofilm within 45 min without ultrasound; however, when insonated (1.5 W/cm2) for 45 min, the disks collected more than 0.45 microg antibiotic. Ultrasonication significantly increased transport of gentamicin across biofilms that normally blocked or slowed gentamicin transport when not exposed to ultrasound. This enhanced transport may be partially responsible for the increased killing of biofilm bacteria exposed to combinations of antibiotic and ultrasound.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.