ArticleTAG. Theoretical and applied genetics. Theoretische und angewandte Genetik2004
Construction of a subgenomic BAC library specific for chromosomes 1D, 4D and 6D of hexaploid wheat.
Article in TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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17 citing papers in PubMed.
- Wheat improvement through advances in single nucleotide polymorphism (SNP) detection and genotyping with a special emphasis on rust resistance.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2024Review
- Haplotype divergence and multiple candidate genes at Rphq2, a partial resistance QTL of barley to Puccinia hordei.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2016Article
- Construction and characterization of a bacterial artificial chromosome library for the hexaploid wheat line 92R137.BioMed research international · 2014Article
- Fine genetic mapping of greenbug aphid-resistance gene Gb3 in Aegilops tauschii.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2012Article
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- Compact genomes and complex evolution in the genus Brachypodium.Chromosoma · 2011Article
- BAC libraries from wheat chromosome 7D: efficient tool for positional cloning of aphid resistance genes.Journal of biomedicine & biotechnology · 2011Article
- Isolated chromosomes as a new and efficient source of DArT markers for the saturation of genetic maps.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2010Article
- Construction and characterization of a bacterial artificial chromosome library for the A-genome of cotton (G. arboreum L.).Journal of biomedicine & biotechnology · 2010Article
- Coupling amplified DNA from flow-sorted chromosomes to high-density SNP mapping in barley.BMC genomics · 2008Article
- A novel resource for genomics of Triticeae: BAC library specific for the short arm of rye (Secale cereale L.) chromosome 1R (1RS).BMC genomics · 2008Article
- Wheat genomics: present status and future prospects.International journal of plant genomics · 2008Article
- Chromosome-based genomics in the cereals.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2007Review
- Dissection of the nuclear genome of barley by chromosome flow sorting.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2006Article
- An alternative to radiation hybrid mapping for large-scale genome analysis in barley.Molecular genetics and genomics : MGG · 2005Article
- A 'Chinese Spring' wheat (Triticum aestivum L.) bacterial artificial chromosome library and its use in the isolation of SSR markers for targeted genome regions.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2005Article
- Article
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12 authors.
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Abstract
The analysis of the hexaploid wheat genome (Triticum aestivum L., 2 n=6 x=42) is hampered by its large size (16,974 Mb/1C) and presence of three homoeologous genomes (A, B and D). One of the possible strategies is a targeted approach based on subgenomic libraries of large DNA inserts. In this work, we purified by flow cytometry a total of 10(7) of three wheat D-genome chromosomes: 1D, 4D and 6D. Chromosomal DNA was partially digested with HindIII and used to prepare a specific bacterial artificial chromosome (BAC) library. The library (designated as TA-subD) consists of 87,168 clones, with an average insert size of 85 kb. Among these clones, 53% had inserts larger than 100 kb, only 29% of inserts being shorter than 75 kb. The coverage was estimated to be 3.4-fold, giving a 96.5% probability of identifying a clone corresponding to any sequence on the three chromosomes. Specificity for chromosomes 1D, 4D and 6D was confirmed after screening the library pools with single-locus microsatellite markers. The screening indicated that the library was not biased and gave an estimated coverage of sixfold. This is the second report on BAC library construction from flow-sorted plant chromosomes, which confirms that dissecting of the complex wheat genome and preparation of subgenomic BAC libraries is possible. Their availability should facilitate the analysis of wheat genome structure and evolution, development of cytogenetic maps, construction of local physical maps and map-based cloning of agronomically important genes.
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