ArticleDiabetologia2004
Rosiglitazone ameliorates insulin resistance in brown adipocytes of Wistar rats by impairing TNF-alpha induction of p38 and p42/p44 mitogen-activated protein kinases.
Article in Diabetologia, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 62 citations in OpenAlex.
- Article
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- Fenofibrate alleviates insulin resistance by reducing tissue inflammation in obese ovariectomized mice.Nutrition & diabetes · 2023Article
- Role of fatty acid transport protein 4 in metabolic tissues: insights into obesity and fatty liver disease.Bioscience reports · 2022Review
- Peroxisome Proliferator-Activated Receptor-Gamma (PPAR-ɣ): Molecular Effects and Its Importance as a Novel Therapeutic Target for Cerebral Ischemic Injury.Neurochemical research · 2021Review
- Farrerol Induces Cancer Cell Death via ERK Activation in SKOV3 Cells and Attenuates TNF-α-Mediated Lipolysis.International journal of molecular sciences · 2021Article
- Towards an Insulin Resistant Adipose Model on a Chip.Cellular and molecular bioengineering · 2021Article
- Coordinated Modulation of Energy Metabolism and Inflammation by Branched-Chain Amino Acids and Fatty Acids.Frontiers in endocrinology · 2020Review
- Astrocyte Resilience to Oxidative Stress Induced by Insulin-like Growth Factor I (IGF-I) Involves Preserved AKT (Protein Kinase B) Activity.The Journal of biological chemistry · 2016Article
- Influencing Factors of Thermogenic Adipose Tissue Activity.Frontiers in physiology · 2016Review
- Identification of nuclear hormone receptor pathways causing insulin resistance by transcriptional and epigenomic analysis.Nature cell biology · 2015Article
- Essential role of protein-tyrosine phosphatase 1B in the modulation of insulin signaling by acetaminophen in hepatocytes.The Journal of biological chemistry · 2014Article
- Insulin, cognition, and dementia.European journal of pharmacology · 2013Review
- In vivo JNK activation in pancreatic β-cells leads to glucose intolerance caused by insulin resistance in pancreas.Diabetes · 2013Article
- Improvement of abnormal liver enzymes after rosiglitazone treatment in Chinese type 2 diabetes.Indian journal of pharmacology · 2012Article
- Rosiglitazone attenuates tumor necrosis factor-α-induced protein-tyrosine phosphatase-1B production in HepG2 cells.Journal of endocrinological investigation · 2012Article
- G protein-coupled receptor kinase 2 plays a relevant role in insulin resistance and obesity.Diabetes · 2010Article
- Inhibitory effect of IL-8 on insulin action in human adipocytes via MAP kinase pathway.Journal of inflammation (London, England) · 2009Article
- Mitochondrial dysfunction and oxidative stress in the pathogenesis of alcohol- and obesity-induced fatty liver diseases.Free radical biology & medicine · 2008Review
- Treatment of non-alcoholic fatty liver disease.Therapeutics and clinical risk management · 2007Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aims/hypothesisTNF-alpha caused insulin resistance on glucose uptake and on insulin signalling in fetal brown adipocytes. Since treatment with TNF-alpha activates stress kinases, including c-jun NH2 terminal kinase (JNK), and p42/p44 and p38 mitogen-activated protein kinases (MAPK), we explored the contribution of these pathways to insulin resistance by TNF-alpha. Rosiglitazone is used to treat Type 2 diabetes as it improves insulin sensitivity in vivo. However, its ability to ameliorate TNF-alpha-induced insulin resistance in brown adipocytes remains to be explored.
methodsWe used fetal rat primary brown adipocytes cultured with TNF-alpha, with or without stress kinase inhibitors or rosiglitazone, and further stimulated with insulin. Then, we measured glucose uptake and GLUT4 translocation. To determine the insulin signalling cascade, we submitted cells to lysis, immunoprecipitation and immunoblotting.
resultsExposure to TNF-alpha for 24 h impairs insulin stimulation of the phosphatidylinositol (PI) 3-kinase activity associated with IRS-2 and Akt activity. Pretreatment with PD98059 or PD169316, which inhibit p42/p44MAPK and p38MAPK respectively, restored insulin signalling and insulin-induced glucose uptake in the presence of TNF-alpha. However, in the presence of SP600125, an inhibitor of JNK, TNF-alpha still produced insulin resistance. Rosiglitazone ameliorated insulin resistance by TNF-alpha in brown adipocytes, restoring completely insulin-stimulated glucose uptake and insulin-induced GLUT4 translocation to plasma membrane in parallel to the insulin signalling cascade IRS-2/PI 3-kinase/Akt. CONCLUSIONS/
interpretationRosiglitazone treatment impaired TNF-alpha activation of p38 and p42/p44MAPK, restoring insulin signalling and leading to normalisation of glucose uptake.
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