ArticleJournal of clinical microbiology2004
Uses of Staphylococcus aureus GeneChips in genotyping and genetic composition analysis.
Article in Journal of clinical microbiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 53 citations in OpenAlex.
- Use of ribotyping to retrospectively identify methicillin-resistant Staphylococcus aureus isolates from phase 3 clinical trials for tigecycline that are genotypically related to community-associated isolates.Antimicrobial agents and chemotherapy · 2005Trial
- Structure of ClfA002 in Complex With Neutralizing Antibody AZD7745 Provides Insight into Its Broad Neutralization Mechanism in Staphylococcus aureus Infection.The Journal of infectious diseases · 2025Article
- Article
- Changes in the Staphylococcus aureus transcriptome during early adaptation to the lung.PloS one · 2012Article
- Recombination and population structure in Salmonella enterica.PLoS genetics · 2011Article
- Inference of the transcriptional regulatory network in Staphylococcus aureus by integration of experimental and genomics-based evidence.Journal of bacteriology · 2011Article
- Development of pooled suppression subtractive hybridization to analyze the pangenome of Staphylococcus aureus.Journal of microbiological methods · 2010Article
- Comparative genomic analysis of European and Middle Eastern community-associated methicillin-resistant Staphylococcus aureus (CC80:ST80-IV) isolates by high-density microarray.Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases · 2009Article
- Characterization of the accessory Sec system of Staphylococcus aureus.Journal of bacteriology · 2008Article
- Comparison of multilocus sequence typing, pulsed-field gel electrophoresis, and antimicrobial susceptibility typing for characterization of Salmonella enterica serotype Newport isolates.Journal of clinical microbiology · 2006Article
- Application of molecular techniques to the study of hospital infection.Clinical microbiology reviews · 2006Review
- Characterizing the effect of the Staphylococcus aureus virulence factor regulator, SarA, on log-phase mRNA half-lives.Journal of bacteriology · 2006Article
- Detection of divergent genes in microbial aCGH experiments.BMC bioinformatics · 2006Article
- Overexpression of genes of the cell wall stimulon in clinical isolates of Staphylococcus aureus exhibiting vancomycin-intermediate- S. aureus-type resistance to vancomycin.Journal of bacteriology · 2006Article
- Characterization of a strain of community-associated methicillin-resistant Staphylococcus aureus widely disseminated in the United States.Journal of clinical microbiology · 2006Article
- Design, validation, and application of a seven-strain Staphylococcus aureus PCR product microarray for comparative genomics.Applied and environmental microbiology · 2005Article
- The agr radiation: an early event in the evolution of staphylococci.Journal of bacteriology · 2005Article
- Multidrug resistance in Staphylococcus aureus due to overexpression of a novel multidrug and toxin extrusion (MATE) transport protein.Antimicrobial agents and chemotherapy · 2005Article
- A novel MATE family efflux pump contributes to the reduced susceptibility of laboratory-derived Staphylococcus aureus mutants to tigecycline.Antimicrobial agents and chemotherapy · 2005Article
- MgrA is a multiple regulator of two new efflux pumps in Staphylococcus aureus.Journal of bacteriology · 2005Article
Corrections and comments
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Authors and funding
12 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Understanding the relatedness of strains within a bacterial species is essential for monitoring reservoirs of antimicrobial resistance and for epidemiological studies. Pulsed-field gel electrophoresis (PFGE), ribotyping, and multilocus sequence typing are commonly used for this purpose. However, these techniques are either nonquantitative or provide only a limited estimation of strain relatedness. Moreover, they cannot extensively define the genes that constitute an organism. In the present study, 21 oxacillin-resistant Staphylococcus aureus (ORSA) isolates, representing eight major ORSA lineages, and each of the seven strains for which the complete genomic sequence is publicly available were genotyped using a novel GeneChip-based approach. Strains were also subjected to PFGE and ribotyping analysis. GeneChip results provided a higher level of discrimination among isolates than either ribotyping or PFGE, although strain clustering was similar among the three techniques. In addition, GeneChip signal intensity cutoff values were empirically determined to provide extensive data on the genetic composition of each isolate analyzed. Using this technology it was shown that strains could be examined for each element represented on the GeneChip, including virulence factors, antimicrobial resistance determinants, and agr type. These results were validated by PCR, growth on selective media, and detailed in silico analysis of each of the sequenced genomes. Collectively, this work demonstrates that GeneChips provide extensive genotyping information for S. aureus strains and may play a major role in epidemiological studies in the future where correlating genes with particular disease phenotypes is critical.
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