ArticleJournal of clinical microbiology2004
Use of an oligonucleotide array for laboratory diagnosis of bacteria responsible for acute upper respiratory infections.
Article in Journal of clinical microbiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 83 citations in OpenAlex.
- A novelJournal of clinical microbiology · 2026Article
- Label-free technique for universal and sequence independent detection of oligonucleotides and nuclease activity.Nucleic acids research · 2025Article
- Exploring the Structural Diversity of DNA Bottlebrush Polymers Using an Oligonucleotide Macromonomer Approach.Macromolecules · 2022Article
- Development of a DNA microarray assay for rapid detection of fifteen bacterial pathogens in pneumonia.BMC microbiology · 2020Article
- Oligonucleotide therapy: An emerging focus area for drug delivery in chronic inflammatory respiratory diseases.Chemico-biological interactions · 2019Review
- Polymer Pen Lithography-Fabricated DNA Arrays for Highly Sensitive and Selective Detection of UnamplifiedPolymers · 2019Article
- Innovative DendrisChipsDiagnostics (Basel, Switzerland) · 2018Article
- Mycoplasma pneumoniae from the Respiratory Tract and Beyond.Clinical microbiology reviews · 2017Review
- Application of Multiplex PCR Coupled with Matrix-Assisted Laser Desorption Ionization-Time of Flight Analysis for Simultaneous Detection of 21 Common Respiratory Viruses.Journal of clinical microbiology · 2015Article
- Detection of group a streptococcal pharyngitis by quantitative PCR.BMC infectious diseases · 2013Article
- A microfluidic chip integrating DNA extraction and real-time PCR for the detection of bacteria in saliva.Lab on a chip · 2013Article
- Bacterial pathogens in the nasopharynx, nasal cavity, and osteomeatal complex during wellness and viral infection.American journal of rhinology & allergy · 2013Article
- DNA microarrays for the diagnosis of infectious diseases.Medecine et maladies infectieuses · 2012Review
- Molecular methods for the detection of Mycoplasma and ureaplasma infections in humans: a paper from the 2011 William Beaumont Hospital Symposium on molecular pathology.The Journal of molecular diagnostics : JMD · 2012Review
- Article
- Acute respiratory infection due to Mycoplasma pneumoniae: current status of diagnostic methods.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2010Review
- Detection of tmRNA molecules on microarrays at low temperatures using helper oligonucleotides.BMC biotechnology · 2010Article
- Development of a novel DNA microarray to detect bacterial pathogens in patients with chronic obstructive pulmonary disease (COPD).Journal of microbiological methods · 2010Article
- Rapid identification of bacterial pathogens using a PCR- and microarray-based assay.BMC microbiology · 2009Article
- Molecular detection of Streptococcus pyogenes and Streptococcus dysgalactiae subsp. equisimilis.Molecular biotechnology · 2009Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We developed a diagnostic array of oligonucleotide probes targeting species-specific variable regions of the genes encoding topoisomerases GyrB and ParE of respiratory bacterial pathogens. Suitable broad-range primer sequences were designed based on alignment of gyrB/parE sequences from nine different bacterial species. These species included Corynebacterium diphtheriae, Fusobacterium necrophorum, Haemophilus influenzae, Legionella pneumophila, Moraxella catarrhalis, Mycoplasma pneumoniae, Staphylococcus aureus, Streptococcus pneumoniae, and Streptococcus pyogenes. Specific probe sequences were selected by comparative analysis against the European Bioinformatics Database, as well as gyrB/parE sequences generated for this study. To verify specificity, at least six initial oligonucleotide probe sequences per bacterial species were tested by hybridization on a solid glass support using culture collection strains as templates. Finally, three oligonucleotide probes per bacterial species were utilized to examine 65 middle ear fluid and 29 throat swab samples. The sensitivities of the developed assay compared to classic culture from middle ear fluid samples for H. influenzae, M. catarrhalis, and S. pneumoniae were 96 (93 for culture), 73 (93 for culture), and 100% (78% for culture), respectively. No cross-reactivity with bacterial species belonging to the normal oral flora was observed when the 29 throat swab samples were studied. The sensitivity of the assay to detect S. pyogenes from these samples was 93% (80% for culture). These results provide a proof of concept for the diagnostic use of microarray technology based on broad-range topoisomerase gene amplification, followed by hybridization and specific detection of bacterial species.
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