ArticleJournal of clinical microbiology2004
Development and validation of a diagnostic DNA microarray to detect quinolone-resistant Escherichia coli among clinical isolates.
Article in Journal of clinical microbiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Ciprofloxacin, amoxicillin, and aminoglycosides stimulate genetic and phenotypic changes in uropathogenic Escherichia coli strains.Virulence · 2019Article
- The genetic background of antibiotic resistance among clinical uropathogenic Escherichia coli strains.Molecular biology reports · 2018Article
- Molecular Methods for Detection of Antimicrobial Resistance.Microbiology spectrum · 2017Review
- Solid and Suspension Microarrays for Microbial Diagnostics.Methods in microbiology · 2015Article
- Recent advances in bacteriophage based biosensors for food-borne pathogen detection.Sensors (Basel, Switzerland) · 2013Review
- Development and validation of a resistance and virulence gene microarray targeting Escherichia coli and Salmonella enterica.Journal of microbiological methods · 2010Article
- Use of optical mapping to sort uropathogenic Escherichia coli strains into distinct subgroups.Microbiology (Reading, England) · 2010Article
- Development of a DNA microarray to detect antimicrobial resistance genes identified in the National Center for Biotechnology Information database.Microbial drug resistance (Larchmont, N.Y.) · 2010Article
- Integrated detection of extended-spectrum-beta-lactam resistance by DNA microarray-based genotyping of TEM, SHV, and CTX-M genes.Journal of clinical microbiology · 2010Article
- Basic concepts of microarrays and potential applications in clinical microbiology.Clinical microbiology reviews · 2009Review
- Rapid detection of intestinal pathogens in fecal samples by an improved reverse dot blot method.World journal of gastroenterology · 2009Article
- Multiplex asymmetric PCR-based oligonucleotide microarray for detection of drug resistance genes containing single mutations in Enterobacteriaceae.Antimicrobial agents and chemotherapy · 2007Article
- Identification of human pathogens isolated from blood using microarray hybridisation and signal pattern recognition.BMC microbiology · 2007Article
- Development and validation of an oligonucleotide microarray for detection of multiple virulence and antimicrobial resistance genes in Escherichia coli.Applied and environmental microbiology · 2006Article
- The ABRF MARG microarray survey 2005: taking the pulse of the microarray field.Journal of biomolecular techniques : JBT · 2006Article
- Different modes of action of naphthyridones in gram-positive and gram-negative bacteria.Antimicrobial agents and chemotherapy · 2006Article
- Development of a DNA microarray for detection and identification of fungal pathogens involved in invasive mycoses.Journal of clinical microbiology · 2005Article
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The incidence of resistance against fluoroquinolones among pathogenic bacteria has been increasing in accordance with the worldwide use of this drug. Escherichia coli is one of the most relevant species for quinolone resistance. In this study, a diagnostic microarray for single-base-mutation detection was developed, which can readily identify the most prevalent E. coli genotypes leading to quinolone resistance. Based on genomic sequence analysis using public databases and our own DNA sequencing results, two amino acid positions (83 and 87) on the A subunit of the DNA gyrase, encoded by the gyrA gene, have been identified as mutation hot spots and were selected for DNA microarray detection. Oligonucleotide probes directed against these two positions were designed so that they could cover the most important resistance-causing and silent mutations. The performance of the array was validated with 30 clinical isolates of E. coli from four different hospitals in Germany. The microarray results were confirmed by standard DNA sequencing and were in full agreement with phenotypic antimicrobial susceptibility testing.
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