ArticleJournal of clinical microbiology2004
Rapid detection and identification of human adenovirus species by adenoplex, a multiplex PCR-enzyme hybridization assay.
Article in Journal of clinical microbiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Comparison of sinonasal symptoms in upper respiratory tract infections during the infectious diseases season of November 2023 to March 2024-a cross-sectional study.Frontiers in medicine · 2024Article
- Highly Sensitive Multiplex Detection of Molecular Biomarkers Using Hybridization Chain Reaction in an Encoded Particle Microfluidic Platform.Biosensors · 2023Article
- Versatile nanorobot hand biosensor for specific capture and ultrasensitive quantification of viral nanoparticles.Materials today. Bio · 2022Article
- Development of a Rapid Immuno-Based Screening Assay for the Detection of Adenovirus in Eye Infections.ACS omega · 2022Article
- Immunogenicity of a new, inactivated canine adenovirus type 2 vaccine for dogs.Clinical and experimental vaccine research · 2020Article
- Diagnosis of Pediatric Acute Adenovirus Infections: Is a Positive PCR Sufficient?The Pediatric infectious disease journal · 2016Article
- A Novel Automatic Rapid Diagnostic Test Reader Platform.Computational and mathematical methods in medicine · 2016Article
- Evaluation of a multiplex real-time PCR assay for the detection of respiratory viruses in clinical specimens.Annals of laboratory medicine · 2012Article
- Update on influenza diagnostics: lessons from the novel H1N1 influenza A pandemic.Clinical microbiology reviews · 2012Review
- Real-time qualitative PCR for 57 human adenovirus types from multiple specimen sources.Journal of clinical microbiology · 2012Article
- Real-time PCR with an internal control for detection of all known human adenovirus serotypes.Journal of clinical microbiology · 2008Article
- Detection of respiratory viruses by molecular methods.Clinical microbiology reviews · 2008Review
- Detection of 11 common viral and bacterial pathogens causing community-acquired pneumonia or sepsis in asymptomatic patients by using a multiplex reverse transcription-PCR assay with manual (enzyme hybridization) or automated (electronic microarray) detection.Journal of clinical microbiology · 2008Article
- RespiFinder: a new multiparameter test to differentially identify fifteen respiratory viruses.Journal of clinical microbiology · 2008Article
- Multiplexed reverse transcriptase PCR assay for identification of viral respiratory pathogens at the point of care.Journal of clinical microbiology · 2007Article
- Nucleic acid amplification tests for detection of respiratory viruses.Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology · 2007Review
- High-throughput, sensitive, and accurate multiplex PCR-microsphere flow cytometry system for large-scale comprehensive detection of respiratory viruses.Journal of clinical microbiology · 2007Article
- Chapter 13 Recent Advances and Future Needs in Environmental Virology.Perspectives in medical virology · 2007Review
- Phylogenetic analysis of the main neutralization and hemagglutination determinants of all human adenovirus prototypes as a basis for molecular classification and taxonomy.Journal of virology · 2005Article
- Rapid identification of human adenovirus types 3 and 7 from respiratory specimens via multiplex type-specific PCR.Journal of clinical microbiology · 2005Article
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Human adenoviruses (AdV) have been implicated in a wide variety of diseases and are ubiquitous in populations worldwide. These agents are of concern particularly in immunocompromised patients, children, and military recruits, resulting in severe disease or death. Clinical diagnosis of AdV is usually achieved through routine viral cell culture, which can take weeks for results. Immunofluorescence and enzyme-linked immunosorbent assay-based techniques are more timely but lack sensitivity. The ability to distinguish between the six different AdV species (A to F) is diagnostically relevant, as infections with specific AdV species are often associated with unique clinical outcomes and epidemiological features. Therefore, we developed a multiplex PCR-enzyme hybridization assay, the Adenoplex, using primers to the fiber gene that can simultaneously detect all six AdV species A through F in a single test. The limit of detection (LOD) based on the viral 50% tissue culture infective dose/ml for AdV A, B, C, D, E, and F was 10(-2), 10(-1), 10(-1), 10(-2), 10(-1), and 10(-2), respectively. Similarly, the LOD for the six DNA controls ranged from 10(2) to 10(3) copies/ml. Twelve common respiratory pathogens were tested with the Adenoplex, and no cross-reactivity was observed. We also validated our assay using clinical specimens spiked with different concentrations of AdV strains of each species type and tested by multiplex PCR and culture. The results demonstrated an overall sensitivity and specificity of Adenoplex of 100%. This assay can be completed in as few as 5 h and provides a rapid, specific, and sensitive method to detect and subtype AdV species A through F.
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