ArticleJournal of clinical microbiology2004
Identification to the species level and differentiation between strains of Aspergillus clinical isolates by automated repetitive-sequence-based PCR.
Article in Journal of clinical microbiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
26 citing papers in PubMed, 78 citations in OpenAlex.
- Molecular Characterization ofPolish journal of microbiology · 2022Article
- Development of a Novel Diagnostic Tool for Cercospora Species Based on BOX-PCR System.Current microbiology · 2022Article
- Long-Term Rasamsonia argillacea Complex Species Colonization Revealed by PCR Amplification of Repetitive DNA Sequences in Cystic Fibrosis Patients.Journal of clinical microbiology · 2016Article
- Conventional Morphology Versus PCR Sequencing, rep-PCR, and MALDI-TOF-MS for Identification of Clinical Aspergillus Isolates Collected Over a 2-Year Period in a University Hospital at Kayseri, Turkey.Journal of clinical laboratory analysis · 2016Article
- Molecular epidemiology of Cryptococcus neoformans and Cryptococcus gattii in China between 2007 and 2013 using multilocus sequence typing and the DiversiLab system.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2015Article
- Clustering of clinical and environmental Escherichia coli O104 isolates using the DiversiLab™ repetitive sequence-based PCR system.Current microbiology · 2015Article
- Emerging Trichosporon asahii in elderly patients: epidemiological and molecular analysis by the DiversiLab system.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2014Article
- Aspergillus monitoring project in a large educational hospital using molecular assay.African journal of infectious diseases · 2014Article
- Development of RFLP-PCR method for the identification of medically important Aspergillus species using single restriction enzyme MwoI.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2014Article
- Microsporum canis infection in three familial cases with tinea capitis and tinea corporis.Mycopathologia · 2013Article
- Comparative evaluation of an automated repetitive-sequence-based PCR instrument versus pulsed-field gel electrophoresis in the setting of a Serratia marcescens nosocomial infection outbreak.Journal of clinical microbiology · 2010Article
- Outbreak of intestinal infection due to Rhizopus microsporus.Journal of clinical microbiology · 2009Article
- Comparison of typing results obtained for methicillin-resistant Staphylococcus aureus isolates with the DiversiLab system and pulsed-field gel electrophoresis.Journal of clinical microbiology · 2009Article
- Endemic linezolid-resistant Staphylococcus epidermidis in a critical care unit.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2009Article
- Genetic variation of Phoma sorghina isolates from Southern Africa and Texas.Folia microbiologica · 2009Article
- Repetitive-sequence-based PCR using the DiversiLab system for identification of Aspergillus species.Journal of clinical microbiology · 2008Article
- DNA microarray-based detection and identification of fungal pathogens in clinical samples from neutropenic patients.Journal of clinical microbiology · 2007Article
- Genotypic analysis of invasive Streptococcus pneumoniae from Mali, Africa, by semiautomated repetitive-element PCR and pulsed-field gel electrophoresis.Journal of clinical microbiology · 2007Article
- Identification of Histoplasma capsulatum, Blastomyces dermatitidis, and Coccidioides species by repetitive-sequence-based PCR.Journal of clinical microbiology · 2006Article
- Comparison of an automated repetitive sequence-based PCR microbial typing system to pulsed-field gel electrophoresis for analysis of outbreaks of methicillin-resistant Staphylococcus aureus.Journal of clinical microbiology · 2005Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A commercially available repetitive-sequence-based PCR (rep-PCR) DNA fingerprinting assay adapted to an automated format, the DiversiLab system, enables rapid microbial identification and strain typing. We explored the performance of the DiversiLab system as a molecular typing tool for 69 Aspergillus isolates (38 A. fumigatus, 15 A. flavus, and 16 A. terreus isolates) had been previously characterized by morphological analysis. Initially, 27 Aspergillus isolates (10 A. fumigatus, 9 A. flavus, and 8 A. terreus isolates) were used as controls to create a rep-PCR-based DNA fingerprint library with the DiversiLab software. Then, 42 blinded Aspergillus isolates were typed using the system. The rep-PCR-based profile revealed 98% concordance with morphology-based identification. rep-PCR-based DNA fingerprints were reproducible and were consistent for DNA from both hyphae and conidia. DiversiLab dendrogram reports correctly identified all A. fumigatus (n = 28), A. terreus (n = 8), and A. flavus (n = 6) isolates in the 42 blinded Aspergillus isolates. rep-PCR-based identification of all isolates was 100% in agreement with the contiguous internal transcribed spacer (ITS) region (ITS1-5.8S-ITS2) sequence-based identification of the respective isolates. Additionally, the DiversiLab system could demonstrate strain-level differentiation of A. flavus and A. terreus. Automated rep-PCR may be a time-efficient, effective, easy-to-use, novel genotyping tool for identifying and determining the strain relatedness of fungi. This system may be useful for epidemiological studies, molecular typing, and surveillance of Aspergillus species.
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