ArticleJournal of clinical virology : the official publication of the Pan American Society for Clinical Virology2004
Development of a PCR-and hybridization-based assay (PCR Adenovirus Consensus) for the detection and the species identification of adenoviruses in respiratory specimens.
Article in Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 46 citations in OpenAlex.
- A Novel Multiplex LAMP Assay for the Detection of Respiratory Human Adenoviruses.International journal of molecular sciences · 2024Article
- Comparison of Three Adenovirus Quantitative PCR Assays with ATCC Reference Strains and Clinical Samples.Journal of clinical microbiology · 2019Article
- Adenovirus infections in immunocompetent and immunocompromised patients.Clinical microbiology reviews · 2014Review
- Comparison of in-house real-time quantitative PCR to the Adenovirus R-Gene kit for determination of adenovirus load in clinical samples.Journal of clinical microbiology · 2010Article
- Development of duplex real-time PCR for detection of two DNA respiratory viruses.Journal of virological methods · 2009Article
- Article
- Real-time PCR with an internal control for detection of all known human adenovirus serotypes.Journal of clinical microbiology · 2008Article
- Development of multiplexed real-time quantitative polymerase chain reaction assay for detecting human adenoviruses.Diagnostic microbiology and infectious disease · 2008Article
- Adenoviruses in immunocompromised hosts.Clinical microbiology reviews · 2008Review
- Detection of respiratory viruses by molecular methods.Clinical microbiology reviews · 2008Review
- [Virology: the contribution of molecular biology in the microbiologic diagnosis of pediatric respiratory diseases].Archives de pediatrie : organe officiel de la Societe francaise de pediatrie · 2007Article
- Currently used nucleic acid amplification tests for the detection of viruses and atypicals in acute respiratory infections.Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology · 2007Review
- Nucleic acid amplification tests for detection of respiratory viruses.Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology · 2007Review
- [Current techniques used for the diagnosis of respiratory virus infectious in intensive care units].Reanimation : journal de la Societe de reanimation de langue francaise · 2007Review
- Comparison of multiplex PCR assays and conventional techniques for the diagnostic of respiratory virus infections in children admitted to hospital with an acute respiratory illness.Journal of medical virology · 2006Article
- Typing of human adenoviruses in specimens from immunosuppressed patients by PCR-fragment length analysis and real-time quantitative PCR.Journal of clinical microbiology · 2006Article
- Co-infections of adenovirus species in previously vaccinated patients.Emerging infectious diseases · 2006Article
- Use of PCR to demonstrate presence of adenovirus species B, C, or F as well as coinfection with two adenovirus species in children with flu-like symptoms.Journal of clinical microbiology · 2006Article
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Authors and funding
11 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundAntigen detection assays and viral isolation techniques are routinely used to detect adenoviruses (Ad) associated with respiratory infections, and the value of the polymerase chain reaction (PCR) has recently been assessed.
objectivesThis paper describes a PCR-hybridization-immunoenzymatic assay (PCR Adenovirus consensus) used to detect Ad and identify Ad species in respiratory specimens.
resultsOn seven representative serotypes Ad 12, Ad 3, Ad 7, Ad 11, Ad 1, Ad 8, Ad 4, the mean genome equivalents per ml and the mean 50% infectious doses per ml were 10(6.3)and 10(4), respectively. Using 362 nasal aspirates from children, Ad were detected by immunofluorescence (IF) and culture in 97 cases (27%), by the PCR-Ad hexon method in 107 cases (29.5%) and by the PCR Adenovirus Consensus method in 113 cases (31.2%); 13 samples were found positive by both PCR and negative by the IF and culture methods; five samples were only positive according to the PCR Adenovirus Consensus) method. The sensitivity, specificity, predictive positive value and predictive negative value of the PCR Adenovirus Consensus method were 97.9%, 93.2%, 84%, 99.1%, respectively. The method identified the species (sp) from 91 positive amplicons: 1 Ad sp A, 44 Ad sp B, 42 Ad sp C, 3 Ad sp E, and 1 Ad sp F; 85 isolates were identified by IF or the neutralisation in culture, and 86 by a PCR-RE digestion method. The PCR Adenovirus Consensus detected six positive samples that were negative according to the IF and culture methods, and it identified the precise species of nine IF-positive and culture-negative nasal aspirates.
conclusionThe PCR Adenovirus Consensus technique is more efficient than the classical IF or culture techniques for the detection of Ad in respiratory samples. An internal control is included to validate the screening results, and specific probes are used to identify the Ad species.
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