ArticleThe Biochemical journal2005
Secondary structure, conformational stability and glycosylation of a recombinant Candida rugosa lipase studied by Fourier-transform infrared spectroscopy.
Article in The Biochemical journal, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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23 citing papers in PubMed.
- Design of Experiments Investigation of Sericin Acetylation Using a Quantitative FTIR Approach.Polymers · 2026Article
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- Heterotrophic denitrification for the simultaneous reduction of nitrates using acorn cups as an energy source.Scientific reports · 2025Article
- Spectroscopic analyses for possible transport of polyaromatic hydrocarbons onto fish in Mediterranean Sea.Scientific reports · 2025Article
- Enzyme-Support Interactions ofACS applied materials & interfaces · 2025Article
- Silk Sericin-Based Electrospun Nanofibers Forming Films for Cosmetic Applications: Preparation, Characterization, and Efficacy Evaluation.Molecules (Basel, Switzerland) · 2025Article
- Enhanced Biodiesel Production with Eversa Transform 2.0 Lipase on Magnetic Nanoparticles.Langmuir : the ACS journal of surfaces and colloids · 2024Article
- Probing Blood Plasma Protein Glycosylation with Infrared Spectroscopy.Analytical chemistry · 2024Article
- Experimental methods to study the structure and dynamics of intrinsically disordered regions in proteins.Current research in structural biology · 2024Review
- Exploring diabesity pathophysiology through proteomic analysis usingFrontiers in endocrinology · 2024Article
- Immobilization of Kluyveromyces lactis β-Galactosidase on Meso-macroporous Silica: Use of Infrared Spectroscopy to Rationalize the Catalytic Efficiency.ChemPlusChem · 2022Article
- Determination of Graphene Oxide Adsorption Space by Lysozyme Uptake─Mechanistic Studies.The journal of physical chemistry. B · 2022Article
- Characterization of the Conformational Properties of Soluble and Insoluble Proteins by Fourier Transform Infrared Spectroscopy.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Influence of Maillard reaction conditions and solvent extraction on the surface activity and foaming characteristics of black cumin protein concentrates.Journal of food science and technology · 2021Article
- Frozen Microemulsions for MAPLE Immobilization of Lipase.Molecules (Basel, Switzerland) · 2017Article
- A reverse micelle strategy for fabricating magnetic lipase-immobilized nanoparticles with robust enzymatic activity.Scientific reports · 2017Article
- Controlling enzymatic activity by immobilization on graphene oxide.Die Naturwissenschaften · 2017Article
- Biosensor Applications of MAPLE Deposited Lipase.Biosensors · 2014Article
- Neoglucosylated collagen matrices drive neuronal cells to differentiate.ACS chemical neuroscience · 2014Article
- Reciprocal influence of protein domains in the cold-adapted acyl aminoacyl peptidase from Sporosarcina psychrophila.PloS one · 2013Article
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5 authors.
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Abstract
The secondary structure of lipase 1 from Candida rugosa, a model system for large monomeric enzymes, has been studied by FTIR (Fourier-transform infrared) spectroscopy in water and 2H2O. The secondary structure content, determined by the analysis of the amide I band absorption through second derivative and curve fitting procedures, is in agreement with that estimated by X-ray data and predicts, in addition, the existence of two classes of alpha-helices. We have also investigated the enzyme stability and aggregation at high temperature by following the protein unfolding. The thermal stability determined by FTIR is in excellent agreement with the temperature dependence of the lipase activity. Furthermore, new insights on the glycosylation of the recombinant protein produced in Pichia pastoris and on its heterogeneity related to different fermentation batches were obtained by the analysis of the IR absorption in the 1200-900 cm(-1) carbohydrate region. A drastic reduction of the intensity of this band was found after enzymic deglycosylation of the protein. To confirm that the FTIR absorption in the 1200-900 cm(-1) region depends on the carbohydrate content and glycoform distribution, we performed an MS analysis of the protein sugar moieties. Glycosidic structures of the high mannose type were found, with mannoses ranging from 8 to 25 residues.
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