ArticleMethods in molecular biology (Clifton, N.J.)2005
Isolation and culture of skeletal muscle myofibers as a means to analyze satellite cells.
Article in Methods in molecular biology (Clifton, N.J.), 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 75 papers.
What it found
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Who cites it
75 citing papers in PubMed, 71 citations in OpenAlex.
- Dystrophins DP71 and DP427 determine cell viability during proliferation and myofibre differentiation.Cell death & disease · 2026Article
- Oxaloacetate and Ketone Bodies Synergistically Promote Myoblast Differentiation in L6 Cells.Molecules (Basel, Switzerland) · 2025Article
- Myotube Formation and Cellular Fusion Are Diminished Due to Low Birth Weight in Piglets.International journal of molecular sciences · 2025Article
- Myofibers cultured in viscoelastic hydrogels reveal the effects of integrin-binding and mechanosensing on muscle satellite cells.Acta biomaterialia · 2025Article
- Derivation and long-term maintenance of porcine skeletal muscle progenitor cells.Scientific reports · 2024Article
- Generation of Bidimensional and Three-Dimensional Muscle Culture Systems.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Endogenous bioluminescent reporters reveal a sustained increase in utrophin gene expression upon EZH2 and ERK1/2 inhibition.Communications biology · 2023Article
- Single cell RNA-seq analysis of the flexor digitorum brevis mouse myofibers.Skeletal muscle · 2021Article
- Analysis of human satellite cell dynamics on cultured adult skeletal muscle myofibers.Skeletal muscle · 2021Article
- Excess Glucose Impedes the Proliferation of Skeletal Muscle Satellite Cells Under Adherent Culture Conditions.Frontiers in cell and developmental biology · 2021Article
- Splicing Factor SRSF1 Is Essential for Satellite Cell Proliferation and Postnatal Maturation of Neuromuscular Junctions in Mice.Stem cell reports · 2020Article
- Isolation and ex vivo cultivation of single myofibers from porcine muscle.In vitro cellular & developmental biology. Animal · 2020Article
- Isolation, Culture, and Differentiation of Primary Myoblasts Derived from Muscle Satellite Cells.Bio-protocol · 2020Article
- The primary cilium dampens proliferative signaling and represses a G2/M transcriptional network in quiescent myoblasts.BMC molecular and cell biology · 2020Article
- Matrix Metalloproteinase 13 from Satellite Cells is Required for Efficient Muscle Growth and Regeneration.Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology · 2020Article
- A multiplexed in vitro assay system for evaluating human skeletal muscle functionality in response to drug treatment.Biotechnology and bioengineering · 2020Article
- Hemojuvelin is a novel suppressor for Duchenne muscular dystrophy and age-related muscle wasting.Journal of cachexia, sarcopenia and muscle · 2019Article
- EGFR-Aurka Signaling Rescues Polarity and Regeneration Defects in Dystrophin-Deficient Muscle Stem Cells by Increasing Asymmetric Divisions.Cell stem cell · 2019Article
- Requirement of Pitx2 for skeletal muscle homeostasis.Developmental biology · 2019Article
- β-arrestin 1 regulates β2-adrenergic receptor-mediated skeletal muscle hypertrophy and contractility.Skeletal muscle · 2018Article
15 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
Abstract
Myofibers are the functional contractile units of skeletal muscle. Mononuclear satellite cells located between the basal lamina and the plasmalemma of the myofiber are the primary source of myogenic precursor cells in postnatal muscle. This chapter describes protocols used in our laboratory for isolation, culturing, and immunostaining of single myofibers from mouse skeletal muscle. The isolated myofibers are intact and retain their associated satellite cells underneath the basal lamina. The first protocol discusses myofiber isolation from the flexor digitorum brevis (FDB) muscle. Myofibers are cultured in dishes coated with Vitrogen collagen, and satellite cells remain associated with the myofibers undergoing proliferation and differentiation on the myofiber surface. The second protocol discusses the isolation of longer myofibers from the extensor digitorum longus (EDL). Different from the FDB myofibers, the longer EDL myofibers tend to tangle and break when cultured together; therefore, EDL myofibers are cultured individually. These myofibers are cultured in dishes coated with Matrigel. The satellite cells initially remain associated with the myofiber and later migrate away to its vicinity, resulting in extensive cell proliferation and differentiation. These protocols allow studies on the interplay between the myofiber and its associated satellite cells.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.