ArticleCell communication and signaling : CCS2004
Potential cellular conformations of the CCN3(NOV) protein.
Article in Cell communication and signaling : CCS, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 49 citations in OpenAlex.
- A working model for CCN3 C-terminal domain-mediated transcriptional modulation of the plasminogen activation system.Journal of cell communication and signaling · 2026Review
- CCN3 as a moonlighting signaling protein: a potential model for dual nuclear and extracellular functions across the CCN family.Cell communication and signaling : CCS · 2026Review
- Blocking CCN2 Reduces Established Palmar Neuromuscular Fibrosis and Improves Function Following Repetitive Overuse Injury.International journal of molecular sciences · 2023Article
- The TSP-1 domain of the matricellular protein CCN5 is essential for its nuclear localization and anti-fibrotic function.PloS one · 2022Article
- Cellular communication network factor 3 in cartilage development and maintenance.Journal of cell communication and signaling · 2021Review
- CCN3 is dynamically regulated by treatment and disease state in multiple sclerosis.Journal of neuroinflammation · 2020Article
- Blocking CTGF/CCN2 reduces established skeletal muscle fibrosis in a rat model of overuse injury.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2020Article
- Blocking CCN2 Reduces Progression of Sensorimotor Declines and Fibrosis in a Rat Model of Chronic Repetitive Overuse.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2019Article
- Article
- Connective tissue growth factor (CCN2) is a matricellular preproprotein controlled by proteolytic activation.The Journal of biological chemistry · 2018Article
- The concept of the CCN protein family revisited: a centralized coordination network.Journal of cell communication and signaling · 2018Review
- Article
- Periostin is required for matricellular localization of CCN3 in periodontal ligament of mice.Journal of cell communication and signaling · 2017Article
- CCN3-EZH2-AR feedback loop: new targets for enzalutamide and castration resistant prostate cancer.Journal of cell communication and signaling · 2017Article
- Polycomb-Mediated Disruption of an Androgen Receptor Feedback Loop Drives Castration-Resistant Prostate Cancer.Cancer research · 2017Article
- Differential expression of CCN family members CYR611, CTGF and NOV in gastric cancer and their association with disease progression.Oncology reports · 2016Article
- Emerging role of CCN family proteins in tumorigenesis and cancer metastasis (Review).International journal of molecular medicine · 2015Review
- CCN proteins: A centralized communication network.Journal of cell communication and signaling · 2013Article
- Nov/Ccn3, a novel transcriptional target of FoxO1, impairs pancreatic β-cell function.PloS one · 2013Article
- Flaws in the peer-reviewing process : a critical look at a recent paper studying the role of CCN3 in renal cell carcinoma.Journal of cell communication and signaling · 2012Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aimTo study the cellular distribution of CCN3(NOV) and to determine if the carboxyterminus of CCN3 is hidden or masked due to high affinity interactions with other partners. CCN3 was detected using affinity purified antibodies (anti-K19M-AF) as well as a Protein A purified anti-K19M antibodies (anti-K19M IgG) against a C-terminal 19-aminoacid peptide (K19M) of human CCN3 protein. The antibodies were applied in indirect immunofluorescence tests and immunoenzyme assays on glial tumor cell line, G59, and its CCN3-transfected variant G59/540 and the adrenocortical cell line, NCI-H295R.
resultsAnti-K19M-AF antibodies reacted against K19M peptide in ELISA and recognized two bands of 51 kDa and 30 kDa in H295R (adrenocortical carcinoma) cell culture supernatants by immunoblotting. H295R culture supernatants which contained CCN3 as shown by immunoblotting did not react with anti-CCN3 antibodies in liquid phase. Anti-CCN3 antibodies stained the surface membranes of non-permeabilized H295R and cytoplasm in permeabilized H295R cells. Similarly, anti-CCN3 stained surface membranes of G59/540, but did not react with G59 cells. Prominent cytoplasmic staining was observed in G59/540, as well as the cell footprints of G59/540 and H295R were strongly labeled.
conclusionsThe K19M-AF antibody directed against the C-terminal 19-aminoacid peptide of CCN3 recognized the secreted protein under denaturing conditions. However, the C-terminal motif of secreted CCN3 was not accessible to K19M-AF in liquid phase. These anti-CCN3 antibodies stained CCN3 protein which was localized to cytoplasmic stores, cell membranes and extracellular matrix. This would suggest that cytoplasmic and cell membrane bound CCN3 has an exposed C-terminus while secreted CCN3 has a sequestered C-terminus which could be due to interaction with other proteins or itself (dimerization). Thus the K19M-AF antibodies revealed at least two conformational states of the native CCN3 protein.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.