ArticleThe EMBO journal2004
Structure and DNA-binding properties of the cytolysin regulator CylR2 from Enterococcus faecalis.
Article in The EMBO journal, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 43 citations in OpenAlex.
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- Genomic characterisation of nasal isolates of coagulase-negative Staphylococci from healthy medical students reveals novel Staphylococcal cassette chromosome mec elements.Scientific reports · 2025Article
- Virulence and resistance gene analysis of Rothia nasimurium by whole gene sequencing.Scientific reports · 2025Article
- Bibacillin 1: A two-component lantibiotic frombioRxiv : the preprint server for biology · 2024Article
- An Overview of the Factors Involved in Biofilm Production by theInternational journal of molecular sciences · 2023Review
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- Anti-Biofilm and Antibacterial Activities ofAntibiotics (Basel, Switzerland) · 2022Article
- Crystal structure report of the ImmR transcriptional regulator DNA-binding domain of the Bacillus subtilis ICEBs1 transposon.Scientific reports · 2022Article
- The near-symmetry of protein oligomers: NMR-derived structures.Scientific reports · 2020Article
- Enterococcal Genetics.Microbiology spectrum · 2019Review
- Expression of Genes Involved in Bacteriocin Production and Self-Resistance in Lactobacillus brevis 174A Is Mediated by Two Regulatory Proteins.Applied and environmental microbiology · 2018Article
- Crystal structure of an anti-CRISPR protein, AcrIIA1.Nucleic acids research · 2018Article
- Molecular Mechanism of Quorum-Sensing in Enterococcus faecalis: Its Role in Virulence and Therapeutic Approaches.International journal of molecular sciences · 2017Review
- Virulence Plasmids of Nonsporulating Gram-Positive Pathogens.Microbiology spectrum · 2014Review
- Transcriptional analysis of the Streptococcus pyogenes salivaricin locus.Journal of bacteriology · 2014Article
- Review
- Structural models of protein-DNA complexes based on interface prediction and docking.Current protein & peptide science · 2011Article
- Integrated analysis of the conformation of a protein-linked spin label by crystallography, EPR and NMR spectroscopy.Journal of biomolecular NMR · 2011Article
- Direct stimulus perception and transcription activation by a membrane-bound DNA binding protein.Molecular microbiology · 2009Article
- High-resolution structure determination of the CylR2 homodimer using paramagnetic relaxation enhancement and structure-based prediction of molecular alignment.Journal of biomolecular NMR · 2008Article
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Authors and funding
9 authors at 2 institutions in 2 countries.
Funding
Abstract
Enterococcus faecalis is one of the major causes for hospital-acquired antibiotic-resistant infections. It produces an exotoxin, called cytolysin, which is lethal for a wide range of Gram-positive bacteria and is toxic to higher organisms. Recently, the regulation of the cytolysin operon was connected to autoinduction by a quorum-sensing mechanism involving the CylR1/CylR2 two-component regulatory system. We report here the crystal structure of CylR2 and its properties in solution as determined by heteronuclear NMR spectroscopy. The structure reveals a rigid dimer containing a helix-turn-helix DNA-binding motif as part of a five-helix bundle that is extended by an antiparallel beta-sheet. We show that CylR2 is a DNA-binding protein that binds specifically to a 22 bp fragment of the cytolysin promoter region. NMR chemical shift perturbation experiments identify surfaces involved in DNA binding and are in agreement with a model for the CylR2/DNA complex that attributes binding specificity to a complex network of CylR2/DNA interactions. Our results propose a mechanism where repression is achieved by CylR2 obstruction of the promoter preventing biosynthesis of the cytolysin operon transcript.
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