ArticleProceedings of the National Academy of Sciences of the United States of America2004
Amine-synthesizing enzyme N-substituted formamide deformylase: screening, purification, characterization, and gene cloning.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 23 citations in OpenAlex.
- A previously unappreciated class of metal-dependent bile salt hydrolases from the human gut microbiome.bioRxiv : the preprint server for biology · 2026Article
- A class of metallohydrolases expands bile salt hydrolase activity in the gut.bioRxiv : the preprint server for biology · 2026Article
- New function of aldoxime dehydratase: Redox catalysis and the formation of an unexpected product.PloS one · 2017Article
- Natural low-molecular mass organic compounds with oxidase activity as organocatalysts.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- A new synthetic route to N-benzyl carboxamides through the reverse reaction of N-substituted formamide deformylase.Applied and environmental microbiology · 2014Article
- HylA, an alternative hydrolase for initiation of catabolism of the phenylurea herbicide linuron in Variovorax sp. strains.Applied and environmental microbiology · 2013Article
- Crystal structure of aldoxime dehydratase and its catalytic mechanism involved in carbon-nitrogen triple-bond synthesis.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Novel isonitrile hydratase involved in isonitrile metabolism.The Journal of biological chemistry · 2010Article
- AepA of Pectobacterium is not involved in the regulation of extracellular plant cell wall degrading enzymes production.Molecular genetics and genomics : MGG · 2010Article
- Transcriptional regulation of the nitrile hydratase gene cluster in Pseudomonas chlororaphis B23.Journal of bacteriology · 2008Article
- Discovery of a reaction intermediate of aliphatic aldoxime dehydratase involving heme as an active center.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
N-substituted formamide was produced through the hydration of an isonitrile by isonitrile hydratase in the isonitrile metabolism. The former compound was further degraded by a microorganism, strain F164, which was isolated from soil through an acclimatization culture. The N-substituted formamide-degrading microorganism was identified as Arthrobacter pascens. The microbial degradation was found to proceed through an enzymatic reaction, the N-substituted formamide being hydrolyzed to yield the corresponding amine and formate. The enzyme, designated as N-substituted formamide deformylase (NfdA), was purified and characterized. The native enzyme had a molecular mass of approximately 61 kDa and consisted of two identical subunits. It stoichiometrically catalyzed the hydrolysis of N-benzylformamide (an N-substituted formamide) to benzylamine and formate. Of all of the N-substituted formamides tested, N-benzylformamide was the most suitable substrate for the enzyme. However, no amides were accepted as substrates. The gene (nfdA) encoding this enzyme was also cloned. The deduced amino acid sequence of nfdA exhibited the highest overall sequence identity (28%) with those of regulatory proteins among known proteins. Only the N-terminal region (residues 58-72) of NfdA also showed significant sequence identity (27-73%) to that of each member of the amidohydrolase superfamily, although there was no similarity in the overall sequence except in the above limited region.
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