ArticleBMC biotechnology2004
A high-throughput cell migration assay using scratch wound healing, a comparison of image-based readout methods.
Article in BMC biotechnology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 135 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
135 citing papers in PubMed, 368 citations in OpenAlex.
- Overcoming the leptomeningeal seeding of medulloblastoma by targeting HSP70.Translational oncology · 2026Article
- In vitro wound simulation: A high-throughput device for scratch assays.Methods (San Diego, Calif.) · 2026Article
- Integrated safety and microbiota profiling of fulvic acid formulations across in vitro and in vivo models.Scientific reports · 2026Article
- Selecting the optimal cell migration assay: fundamentals and practical guidelines.Nature methods · 2026Review
- pH-responsive bilayer hydrogel with sequential release of morin-based nanoparticles and bFGF for the treatment of the "ice and fire" wounds.Materials today. Bio · 2025Article
- MiR-10a as a Potential Biomarker and Therapeutic Target in Localized and Metastatic Prostate Cancer.Current issues in molecular biology · 2025Article
- In Vitro Wound Simulation: A High-Throughput Device for Scratch Assays.bioRxiv : the preprint server for biology · 2025Article
- PDMS Membranes Drilled by Proton Microbeam Writing: A Customizable Platform for the Investigation of Endothelial Cell-Substrate Interactions in Transwell-like Devices.Journal of functional biomaterials · 2025Article
- Assessment of the toxic effects of parabens, commonly used preservatives in cosmetics, and their halogenated by-products on human skin and endothelial cells.NAM journal · 2025Article
- A programmable, open-source robot that scratches cultured tissues to investigate cell migration, healing, and tissue sculpting.Cell reports methods · 2024Article
- SCRATCH: A programmable, open-hardware, benchtop robot that automatically scratches cultured tissues to investigate cell migration, healing, and tissue sculpting.bioRxiv : the preprint server for biology · 2024Article
- Systemic Dendrimer-Peptide Therapies for Wet Age-Related Macular Degeneration.Pharmaceutics · 2023Article
- FBXW2 suppresses breast tumorigenesis by targeting AKT-Moesin-SKP2 axis.Cell death & disease · 2023Article
- An effective device to enable consistent scratches for in vitro scratch assays.BMC biotechnology · 2023Article
- Cytotoxic activity of essential oil from Leaves of Myrcia splendens against A549 Lung Cancer cells.BMC complementary medicine and therapies · 2023Article
- Inference of glioblastoma migration and proliferation rates using single time-point images.Communications biology · 2023Article
- Mechanisms and modeling of wound repair in the intestinal epithelium.Tissue barriers · 2023Review
- Inhibition of human oral squamous cell carcinoma proliferation and migration by prodrug-activating suicide gene therapies.Experimental and therapeutic medicine · 2023Article
- Tools for studying human microglia: In vitro and in vivo strategies.Brain, behavior, and immunity · 2023Review
- Skin models of cutaneous toxicity, transdermal transport and wound repair.Burns & trauma · 2023Review
75 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 4 institutions in 2 countries.
Funding
Abstract
backgroundCell migration is a complex phenomenon that requires the coordination of numerous cellular processes. Investigation of cell migration and its underlying biology is of interest to basic scientists and those in search of therapeutics. Current migration assays for screening small molecules, siRNAs, or other perturbations are difficult to perform in parallel at the scale required to screen large libraries.
resultsWe have adapted the commonly used scratch wound healing assay of tissue-culture cell monolayers to a 384 well plate format. By mechanically scratching the cell substrate with a pin array, we are able to create characteristically sized wounds in all wells of a 384 well plate. Imaging of the healing wounds with an automated fluorescence microscope allows us to distinguish perturbations that affect cell migration, morphology, and division. Readout requires ~1 hr per plate but is high in information content i.e. high content. We compare readouts using different imaging technologies, automated microscopy, scanners and a fluorescence macroscope, and evaluate the trade-off between information content and data acquisition rate.
conclusionsThe adaptation of a wound healing assay to a 384 well format facilitates the study of aspects of cell migration, tissue reorganization, cell division, and other processes that underlie wound healing. This assay allows greater than 10,000 perturbations to be screened per day with a quantitative, high-content readout, and can also be used to characterize small numbers of perturbations in detail.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.