ArticleThe Biochemical journal1992
Soluble low-Km 5'-nucleotidase from electric-ray (Torpedo marmorata) electric organ and bovine cerebral cortex is derived from the glycosyl-phosphatidylinositol-anchored ectoenzyme by phospholipase C cleavage.
Article in The Biochemical journal, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 70 citations in OpenAlex.
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- CD73 Inhibits cGAS-STING and Cooperates with CD39 to Promote Pancreatic Cancer.Cancer immunology research · 2023Article
- Elevated ATP via enhanced miRNA-30b, 30c, and 30e downregulates the expression of CD73 in CD8+ T cells of HIV-infected individuals.PLoS pathogens · 2022Article
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- Generation and Function of Non-cell-bound CD73 in Inflammation.Frontiers in immunology · 2019Review
- Purine-related metabolites and their converting enzymes are altered in frontal, parietal and temporal cortex at early stages of Alzheimer's disease pathology.Brain pathology (Zurich, Switzerland) · 2018Article
- Cellular function and molecular structure of ecto-nucleotidases.Purinergic signalling · 2012Review
- TRANSLATION OF BRAIN ACTIVITY INTO SLEEP.Hirosaki igaku = Hirosaki medical journal · 2012Article
- Identification of the nucleotidase responsible for the AMP hydrolysing hyperactivity associated with neurological and developmental disorders.Neurochemical research · 2008Article
- Ecto-5'-nucleotidase: Structure function relationships.Purinergic signalling · 2006Article
- Insulin stimulates the release of the glycosyl phosphatidylinositol-anchored membrane dipeptidase from 3T3-L1 adipocytes through the action of a phospholipase C.The Biochemical journal · 1997Article
- Membrane protein secretases.The Biochemical journal · 1997Review
- Glucose-induced sequential processing of a glycosyl-phosphatidylinositol-anchored ectoprotein in Saccharomyces cerevisiae.Molecular and cellular biology · 1996Article
- Activation of the glycosyl-phosphatidylinositol-anchored membrane dipeptidase upon release from pig kidney membranes by phospholipase C.The Biochemical journal · 1994Article
- The structure, biosynthesis and function of glycosylated phosphatidylinositols in the parasitic protozoa and higher eukaryotes.The Biochemical journal · 1993Review
- Glucose induces lipolytic cleavage of a glycolipidic plasma membrane anchor in yeast.The Journal of cell biology · 1993Article
- 5'-Nucleotidase: molecular structure and functional aspects.The Biochemical journal · 1992Review
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Soluble and membrane-bound low-Km 5'-nucleotidase was isolated from high-speed supernatants and membrane fractions derived from the electric organ of the electric ray (Torpedo marmorata) or from bovine brain cerebral cortex. Purification of both enzymes included chromatography on concanavalin A-Sepharose and AMP-Sepharose. The contribution to the total of soluble enzyme activity was lower in electric organ (1.6%) than in bovine cerebral cortex (27.9%). Membrane-bound and soluble forms have very similar Km values for AMP and are inhibited by micromolar concentrations of ATP. Both forms cross-react with, and are inhibited by, an antibody against the membrane-bound surface-located (ecto-) 5'-nucleotidase from electric organ. The HNK-1 carbohydrate epitope is present on both forms of the Torpedo enzyme, but is entirely absent from bovine cerebral-cortex 5'-nucleotidase. An antibody specific for the inositol 1,2-(cyclic)monophosphate that is formed on phospholipase C cleavage of an intact glycosyl-phosphatidylinositol (GPI) anchor binds to the soluble, but not to the membrane-bound, form of the enzyme from both sources. Our results suggest that soluble low-Km 5'-nucleotidase in both electric organ and bovine brain is derived from the membrane-bound GPI-anchored form of the enzyme by the action of a phospholipase C and is not a soluble cytoplasmic enzyme.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.