ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2004
Chloride accumulation in mammalian olfactory sensory neurons.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 88 papers.
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Who cites it
88 citing papers in PubMed, 197 citations in OpenAlex.
- Overactive EAAT1 ClActa neuropathologica communications · 2026Article
- Design principles of ciliary signaling.Journal of cell science · 2025Review
- Prolonged Hyperactivity Elicits Massive and Persistent Chloride Ion Redistribution in Subsets of Cultured Hippocampal Dentate Granule Cells.bioRxiv : the preprint server for biology · 2025Article
- LRRC8 channel complexes counterbalance KATP channels to mediate swell-secretion coupling in mouse pancreatic β cells.JCI insight · 2025Article
- Amplification of Olfactory Transduction Currents Implements Sparse Stimulus Encoding.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2025Article
- Paired and solitary ionocytes in the zebrafish olfactory epithelium.Chemical senses · 2025Article
- Displacement of extracellular chloride by immobile anionic constituents of the brain's extracellular matrix.The Journal of physiology · 2025Article
- All-optical reporting of inhibitory receptor driving force in the nervous system.Nature communications · 2024Article
- Amplification of olfactory transduction currents implements sparse stimulus encoding.bioRxiv : the preprint server for biology · 2024Article
- The CaThe Journal of physiology · 2024Article
- New insights into the roles of olfactory receptors in cardiovascular disease.Molecular and cellular biochemistry · 2024Review
- GABA Type A receptors expressed in triple negative breast cancer cells mediate chloride ion flux.Frontiers in pharmacology · 2024Article
- Function and Regulation of the Calcium-Activated Chloride Channel Anoctamin 1 (TMEM16A).Handbook of experimental pharmacology · 2024Article
- Membrane manipulation by free fatty acids improves microbial plant polyphenol synthesis.Nature communications · 2023Article
- Astrocytic chloride is brain state dependent and modulates inhibitory neurotransmission in mice.Nature communications · 2023Article
- Paradoxical electro-olfactogram responses in TMEM16B knock-out mice.Chemical senses · 2023Article
- Nitric Oxide Sensing by a Blue Fluorescent Protein.Antioxidants (Basel, Switzerland) · 2022Article
- Low signaling efficiency from receptor to effector in olfactory transduction: A quantified ligand-triggered GPCR pathway.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Fluorescence depolarization dynamics of ionic strength sensors using time-resolved anisotropy.Biophysical journal · 2021Article
- Intricacies of GABAInternational journal of molecular sciences · 2021Review
28 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The generation of an excitatory receptor current in mammalian olfactory sensory neurons (OSNs) involves the sequential activation of two distinct types of ion channels: cAMP-gated Ca(2+)-permeable cation channels and Ca(2+)-gated Cl(-) channels, which conduct a depolarizing Cl(-) efflux. This unusual transduction mechanism requires an outward-directed driving force for Cl(-), established by active accumulation of Cl(-) within the lumen of the sensory cilia. We used two-photon fluorescence lifetime imaging microscopy of the Cl(-)-sensitive dye 6-methoxy-quinolyl acetoethyl ester to measure the intracellular Cl(-) concentration in dendritic knobs of OSNs from mice and rats. We found a uniform intracellular Cl(-) concentration in the range of 40-50 mm, which is indicative of active Cl(-) accumulation. Functional assays and PCR experiments revealed that NKCC1-mediated Cl(-) uptake through the apical membrane counteracts Cl(-) depletion in the sensory cilia, and thus maintains the responsiveness of OSNs to odor stimulation. To permit Cl(-) accumulation, OSNs avoid the "chloride switch": they do not express KCC2, the main Cl(-) extrusion cotransporter operating in neurons of the adult CNS. Cl(-) accumulation provides OSNs with the driving force for the depolarizing Cl(-) current that is the basis of the low-noise receptor current in these neurons.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.