ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2004
Functional properties of motoneurons derived from mouse embryonic stem cells.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 102 papers.
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Who cites it
102 citing papers in PubMed, 209 citations in OpenAlex.
- Human motor neurons generated from neural stem cells delay clinical onset and prolong life in ALS mouse model.PloS one · 2014Trial
- Human iPSC-Derived Spinal Cord Organoids - The First Electrophysiological Assessment of Neuronal Maturation.ACS chemical neuroscience · 2026Article
- Direct conversion of urine-derived cells into functional motor neuron-like cells by defined transcription factors.Scientific reports · 2024Article
- Rapid responses of human pluripotent stem cells to cyclic mechanical strains applied to integrin by acoustic tweezing cytometry.Scientific reports · 2023Article
- A scalable human iPSC-based neuromuscular disease model on suspended biobased elastomer nanofiber scaffolds.Biofabrication · 2023Article
- Regenerative medicine improve neurodegenerative diseases.Cell and tissue banking · 2023Review
- Transcriptional dynamics of murine motor neuron maturation in vivo and in vitro.Nature communications · 2022Article
- Electrical Properties of Adult Mammalian Motoneurons.Advances in neurobiology · 2022Article
- Potassium channels and the development of arousal-relevant action potential trains in primary hindbrain neurons.Brain research · 2021Article
- Getting in touch with your senses: Mechanisms specifying sensory interneurons in the dorsal spinal cord.WIREs mechanisms of disease · 2021Review
- Fully Characterized Mature Human iPS- and NMP-Derived Motor Neurons Thrive Without Neuroprotection in the Spinal Contusion Cavity.Frontiers in cellular neuroscience · 2021Article
- Spinal motoneuron firing properties mature from rostral to caudal during postnatal development of the mouse.The Journal of physiology · 2020Article
- Efficient and rapid conversion of human astrocytes and ALS mouse model spinal cord astrocytes into motor neuron-like cells by defined small molecules.Military Medical Research · 2020Article
- Optimized Protocol to Generate Spinal Motor Neuron Cells from Induced Pluripotent Stem Cells from Charcot Marie Tooth Patients.Brain sciences · 2020Article
- PuraMatrix hydrogel enhances the expression of motor neuron progenitor marker and improves adhesion and proliferation of motor neuron-like cells.Iranian journal of basic medical sciences · 2020Article
- Neuromuscular actuation of biohybrid motile bots.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Article
- A Stem Cell-Based Screening Platform Identifies Compounds that Desensitize Motor Neurons to Endoplasmic Reticulum Stress.Molecular therapy : the journal of the American Society of Gene Therapy · 2019Article
- Hindbrain V2a Neurons Pattern Rhythmic Activity of Motor Neurons in a Reticulospinal Coculture.Frontiers in neuroscience · 2019Article
- Establishment of a myelinating co-culture system with a motor neuron-like cell line NSC-34 and an adult rat Schwann cell line IFRS1.Histochemistry and cell biology · 2018Article
42 more citing papers are in PubMed but not listed here.
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The capacity of embryonic stem (ES) cells to form functional motoneurons (MNs) and appropriate connections with muscle was investigated in vitro. ES cells were obtained from a transgenic mouse line in which the gene for enhanced green fluorescent protein (eGFP) is expressed under the control of the promotor of the MN specific homeobox gene Hb9. ES cells were exposed to retinoic acid (RA) and sonic hedgehog agonist (Hh-Ag1.3) to stimulate differentiation into MNs marked by expression of eGFP and the cholinergic transmitter synthetic enzyme choline acetyltransferase. Whole-cell patch-clamp recordings were made from eGFP-labeled cells to investigate the development of functional characteristics of MNs. In voltage-clamp mode, currents, including EPSCs, were recorded in response to exogenous applications of GABA, glycine, and glutamate. EGFP-labeled neurons also express voltage-activated ion channels including fast-inactivating Na(+) channels, delayed rectifier and I(A)-type K(+) channels, and Ca(2+) channels. Current-clamp recordings demonstrated that eGFP-positive neurons generate repetitive trains of action potentials and that l-type Ca(2+) channels mediate sustained depolarizations. When cocultured with a muscle cell line, clustering of acetylcholine receptors on muscle fibers adjacent to developing axons was seen. Intracellular recordings of muscle fibers adjacent to eGFP-positive axons revealed endplate potentials that increased in amplitude and frequency after glutamate application and were sensitive to TTX and curare. In summary, our findings demonstrate that MNs derived from ES cells develop appropriate transmitter receptors, intrinsic properties necessary for appropriate patterns of action potential firing and functional synapses with muscle fibers.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.