ArticleProceedings of the National Academy of Sciences of the United States of America2004
Reverse transcriptase activity innate to DNA polymerase I and DNA topoisomerase I proteins of Streptomyces telomere complex.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 34 citations in OpenAlex.
- DNA polymerase I: structure, activity, and function in bacterial DNA replication and repair.Critical reviews in biochemistry and molecular biology · 2026Review
- Natural History of DNA-Dependent DNA Polymerases: Multiple Pathways to the Origins of DNA.Viruses · 2023Article
- Compaction and control-the role of chromosome-organizing proteins in Streptomyces.FEMS microbiology reviews · 2020Review
- A highly processive actinobacterial topoisomerase I - thoughts onMicrobiology (Reading, England) · 2020Review
- Microbe Profile:Microbiology (Reading, England) · 2019Review
- TRF2-Mediated Control of Telomere DNA Topology as a Mechanism for Chromosome-End Protection.Molecular cell · 2016Article
- A highly processive topoisomerase I: studies at the single-molecule level.Nucleic acids research · 2014Article
- Transgene-mediated co-suppression of DNA topoisomerase-1 gene in Caenorhabditis elegans.International journal of biochemistry and molecular biology · 2014Article
- Telomeric impact of conventional chemotherapy.Frontiers of medicine · 2013Review
- Lateral gene transfer of family A DNA polymerases between thermophilic viruses, aquificae, and apicomplexa.Molecular biology and evolution · 2013Article
- One identity or more for telomeres?Frontiers in oncology · 2013Article
- Linear Streptomyces plasmids form superhelical circles through interactions between their terminal proteins.Nucleic acids research · 2011Article
- Streptomyces telomeres contain a promoter.Journal of bacteriology · 2009Article
- The linear chromosome of the plant-pathogenic mycoplasma 'Candidatus Phytoplasma mali'.BMC genomics · 2008Article
- DNA polymerase I is not required for replication of linear chromosomes in streptomyces.Journal of bacteriology · 2008Article
- Centromeres were derived from telomeres during the evolution of the eukaryotic chromosome.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- In vitro deoxynucleotidylation of the terminal protein of Streptomyces linear chromosomes.Applied and environmental microbiology · 2006Article
- Reverse transcriptase at bacterial telomeres.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
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2 authors at 1 institution in 1 country.
Funding
Abstract
Replication of Streptomyces linear chromosomes and plasmids proceeds bidirectionally from a central origin, leaving recessed 5' termini that are extended by a telomere binding complex. This complex contains both a telomere-protecting terminal protein (Tpg) and a telomere-associated protein that interacts with Tpg and the DNA ends of linear Streptomyces replicons. By using histidine-tagged telomere-associated protein (Tap) as a scaffold, we identified DNA polymerase (PolA) and topoisomerase I (TopA) proteins as other components of the Streptomyces telomere complex. Biochemical characterization of these proteins indicated that both PolA and TopA exhibit highly efficient reverse transcriptase (RT) activity in addition to their predicted functions. Although RT activity innate to other DNA-dependent DNA polymerases has been observed previously, its occurrence in a topoisomerase is unprecedented. Deletion mapping and sequence analysis showed that the RT activity of Streptomcyces TopA resides in a peptide region containing motifs that are absent from most bacterial topoisomerases but are highly conserved in a novel subfamily of eubacterial topoisomerases found largely in Actinobacteria. Within one of these motifs, and essential to the RT function of Streptomyces TopA, is an Asp-Asp doublet sequence required also for the RT activities of human immunodeficiency virus and eukaryotic cell telomerases.
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