ArticleThe Journal of cell biology2004
Phosphorylation of actopaxin regulates cell spreading and migration.
Article in The Journal of cell biology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
24 citing papers in PubMed, 52 citations in OpenAlex.
- Article
- Matrix Remodeling Associated Genes (MXRAs): structural diversity, functional significance, and therapeutic potential in tumor microenvironments.Discover oncology · 2025Review
- Systemic cellular migration: The forces driving the directed locomotion movement of cells.PNAS nexus · 2024Article
- Identification of Phosphorylated Proteins Regulated by SDF2L1 in Nasopharyngeal Carcinoma Cells.Evolutionary bioinformatics online · 2022Article
- Downregulation of PARVA promotes metastasis by modulating integrin-linked kinase activity and regulating MAPK/ERK and MLC2 signaling in prostate cancer.Translational andrology and urology · 2021Article
- α-Parvin promotes breast cancer progression and metastasis through interaction with G3BP2 and regulation of TWIST1 signaling.Oncogene · 2019Article
- α-Parvin and β-parvin in the rat uterus during decidualisation and uterine receptivity.Histochemistry and cell biology · 2019Article
- The Focal-Focal Preconditioning Effect of Photothrombotic Impact on the Signaling Protein Profile in the Penumbra Surrounding the Ischemic Core Induced by Another Photothrombotic Impact.Molecular neurobiology · 2018Article
- Profiling of Signaling Proteins in Penumbra After Focal Photothrombotic Infarct in the Rat Brain Cortex.Molecular neurobiology · 2017Article
- Integrative systems and synthetic biology of cell-matrix adhesion sites.Cell adhesion & migration · 2016Review
- Article
- Actopaxin (α-parvin) phosphorylation is required for matrix degradation and cancer cell invasion.The Journal of biological chemistry · 2012Article
- CdGAP regulates cell migration and adhesion dynamics in two-and three-dimensional matrix environments.Cytoskeleton (Hoboken, N.J.) · 2012Article
- LNK (SH2B3) is a key regulator of integrin signaling in endothelial cells and targets α-parvin to control cell adhesion and migration.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2012Article
- Stages of ciliogenesis and regulation of ciliary length.Differentiation; research in biological diversity · 2012Review
- Beta2-adaptin binds actopaxin and regulates cell spreading, migration and matrix degradation.PloS one · 2012Article
- Paxillin and Hic-5 interaction with vinculin is differentially regulated by Rac1 and RhoA.PloS one · 2012Article
- Role of palladin phosphorylation by extracellular signal-regulated kinase in cell migration.PloS one · 2011Article
- Quantitative phosphoproteome analysis of lysophosphatidic acid induced chemotaxis applying dual-step (18)O labeling coupled with immobilized metal-ion affinity chromatography.Journal of proteome research · 2008Article
- Paxillin comes of age.Journal of cell science · 2008Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Actopaxin is an actin and paxillin binding protein that localizes to focal adhesions. It regulates cell spreading and is phosphorylated during mitosis. Herein, we identify a role for actopaxin phosphorylation in cell spreading and migration. Stable clones of U2OS cells expressing actopaxin wild-type (WT), nonphosphorylatable, and phosphomimetic mutants were developed to evaluate actopaxin function. All proteins targeted to focal adhesions, however the nonphosphorylatable mutant inhibited spreading whereas the phosphomimetic mutant cells spread more efficiently than WT cells. Endogenous and WT actopaxin, but not the nonphosphorylatable mutant, were phosphorylated in vivo during cell adhesion/spreading. Expression of the nonphosphorylatable actopaxin mutant significantly reduced cell migration, whereas expression of the phosphomimetic increased cell migration in scrape wound and Boyden chamber migration assays. In vitro kinase assays demonstrate that extracellular signal-regulated protein kinase phosphorylates actopaxin, and treatment of U2OS cells with the MEK1 inhibitor UO126 inhibited adhesion-induced phosphorylation of actopaxin and also inhibited cell migration.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.