ArticlePlanta2005
Expression of poly-3-(R)-hydroxyalkanoate (PHA) polymerase and acyl-CoA-transacylase in plastids of transgenic potato leads to the synthesis of a hydrophobic polymer, presumably medium-chain-length PHAs.
Article in Planta, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 42 citations in OpenAlex.
- Insights into widespread disturbance in gene expression and severe growth inhibition observed in transgenic rice producing polyhydroxybutyrate.Plant biotechnology (Tokyo, Japan) · 2025Article
- Biotechnological Approaches for Enhancing Polyhydroxyalkanoates (PHAs) Production: Current and Future Perspectives.Current microbiology · 2023Review
- Metabolic engineering strategies to produce medium-chain oleochemicals via acyl-ACP:CoA transacylase activity.Nature communications · 2022Article
- Production and characterization of two medium-chain-length polydroxyalkanoates by engineered strains of Yarrowia lipolytica.Microbial cell factories · 2019Article
- Effect of mcl-PHA synthesis in flax on plant mechanical properties and cell wall composition.Transgenic research · 2019Article
- Poly(-β-hydroxybutyrate) (PHB) depolymerase PHAZ3 Biotech · 2015Article
- Production of (R)-3-hydroxybutyric acid by Burkholderia cepacia from wood extract hydrolysates.AMB Express · 2014Article
- Introduction of the rd29A:AtDREB2A CA gene into soybean (Glycine max L. Merril) and its molecular characterization in leaves and roots during dehydration.Genetics and molecular biology · 2013Article
- Large-scale production of poly(3-hydroxyoctanoic acid) by Pseudomonas putida GPo1 and a simplified downstream process.Applied and environmental microbiology · 2009Article
- Assessment of technological options and economical feasibility for cyanophycin biopolymer and high-value amino acid production.Applied microbiology and biotechnology · 2007Review
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Medium-chain-length poly-3-(R)-hydroxyalkanoates (mcl-PHAs) belong to the group of microbial polyesters. The minimum gene-set for the accumulation of mcl-PHAs from de novo fatty acid biosynthesis has been identified in prokaryotes as consisting of the Pha-C1 polymerase and the ACP-CoA-transacylase. In this paper, the synthesis of mcl-PHAs has been attempted in transgenic potato (Solanum tuberosum L.) using the same set of genes that were introduced into potato by particle bombardment. Polymer contents of transgenic lines were analysed by gas chromatography and by a new simple method employing a size-exclusion filter column. The expression of the Pha-C1 polymerase and the ACP-CoA-transacylase in the plastids of transgenic potato led to the synthesis of a hydrophobic polymer composed of mcl-hydroxy-fatty acids with carbon chain lengths ranging from C-6 to C-12 in leaves of the selected transgenic lines. We strongly suggest that the polymer observed consists of mcl-PHAs and that this report establishes for the first time a possible route for the production of mcl-PHAs from de novo fatty acid biosynthesis in plants.
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