Evidence map›Paper›PMID 15350733›Full record

ArticleJournal of virological methods2004

A universal microarray for detection of SARS coronavirus.

Wei-Hong Long, Hua-Sheng Xiao, Xiao-Mei Gu, Qing-Hua Zhang, Hong-Jun Yang, Guo-Ping Zhao, Jian-Hua Liu

Abstract readEvaluation Study
In one paragraph

Article in Journal of virological methods, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.

0numbers the graph read from it
0cells of the map it votes in
22citing papers in PubMed
2.8field-weighted citation impact, top 10% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

22 citing papers in PubMed, 49 citations in OpenAlex.

  1. Review
  2. Review
  3. Micro and Nanoscale Technologies for Diagnosis of Viral Infections.Small (Weinheim an der Bergstrasse, Germany) · 2021
    Review
  4. Review
  5. Review
  6. Strategies and perspectives to develop SARS-CoV-2 detection methods and diagnostics.Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie · 2020
    Review
  7. Review
  8. Review
  9. Article
  10. Article
  11. Article
  12. Article
  13. Review
  14. Article
  15. Review
  16. Article
  17. Microarray technology in obstetrics and gynecology: a guide for clinicians.American journal of obstetrics and gynecology · 2006
    Review
  18. Article
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Wei-Hong LongSchool of Life Science and Technology, Shanghai JiaoTong University, 1954 Hua-Shan Road, Shanghai 200030, China.
Hua-Sheng Xiao
Xiao-Mei Gu
Qing-Hua Zhang
Hong-Jun Yang
Guo-Ping Zhao
Jian-Hua Liu
Shanghai Jiao Tong University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Severe acute respiratory syndrome (SARS) is caused by the SARS coronavirus (SARS-CoV). There are many point mutations among SARS-CoV genome sequences. Previous studies suggested that the mutations are correlated closely with the SARS epidemic. It was found that the bases of six nucleotide positions (nt9404, nt9479, nt19838, nt21721, nt22222 and nt27827) with high-mutation rate have an important relationship with the SARS epidemic. For viral detection as well as genotyping, a universal microarray system was developed that combines RT-PCR and ligase detection reaction (LDR). The Zip Codes attached covalently to a slide remain constant and their complementary Zip Codes (cZip Codes) can be used for tagging target sequence, making the microarrays universal. The discriminating oligonucleotides contain on the 5' end "cZip Codes" that are used to direct LDR product to specific Zip Codes attached covalently to a slide. Since Zip Codes have no homology to either the target sequence or to other sequences in the genomes of both human host and SARS-CoV, there was no false signal due to mismatch hybridizations. 20 samples assayed with the universal microarray were confirmed by DNA sequencing, demonstrating that this microarray system is a promising diagnostic tool for detection and genotyping of the SARS-CoV.

Indexed as

Molecular Diagnostic TechniquesOligonucleotide Array Sequence AnalysisAmino Acid SubstitutionGenetic VariationGenotypeHumansLigase Chain ReactionMolecular EpidemiologyNucleic Acid HybridizationPoint MutationReverse Transcriptase Polymerase Chain ReactionSevere Acute Respiratory SyndromeSevere acute respiratory syndrome-related coronavirus

Identifiers

PMID15350733
PMCPMC7112900
OpenAlexW2059686858

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.