ArticleAcademic radiology2004
In vivo imaging of MLC2v-luciferase, a cardiac-specific reporter gene expression in mice.
Article in Academic radiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 15 citations in OpenAlex.
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- Tracking stem cells for cardiovascular applications in vivo: focus on imaging techniques.Imaging in medicine · 2011Article
- Embryonic stem cell-derived cardiomyocyte heterogeneity and the isolation of immature and committed cells for cardiac remodeling and regeneration.Stem cells international · 2011Article
- Advances in cardiovascular molecular imaging for tracking stem cell therapy.Thrombosis and haemostasis · 2010Review
- Lentiviral vectors bearing the cardiac promoter of the Na+-Ca2+ exchanger report cardiogenic differentiation in stem cells.Molecular therapy : the journal of the American Society of Gene Therapy · 2008Article
- Imaging stem cells implanted in infarcted myocardium.Journal of the American College of Cardiology · 2006Review
- In vivo detection of stem cells grafted in infarcted rat myocardium.Journal of nuclear medicine : official publication, Society of Nuclear Medicine · 2005Article
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
Abstract
RATIONALE AND
objectivesA reporter or marker gene that is detectable by in vivo imaging permits longitudinal monitoring of certain fundamental biological processes (eg, differentiation) within the context of physiologically authentic environments. Tissue-specific expression of a reporter gene can be achieved when it is under the transcriptional control of a tissue-specific promoter. The objective of this study was to construct a plasmid vector containing firefly luciferase (Fluc) marker gene downstream of the promoter sequence of rat ventricular myosin light chain 2 (MLC2v); to detect the in vivo expression of this cardiac-specific reporter (MLC2v-Fluc) in the mouse heart by bioluminescent imaging; and to correlate the bioluminescent signal with postmortem luminometer assay. MATERIALS AND
methodsMLC2v-Fluc plasmid was generated by molecular cloning of 3 kb promoter sequence into a pGL3-Basic vector containing the Fluc reporter. Twenty microg of MLC2v-Fluc plasmid DNA in phosphate-buffered saline was directly injected into mouse myocardium through a midline sternotomy.
resultsAt 1 week after injection, MLC2v-Fluc expression was detected by in vivo bioluminescent imaging in 60% of injected animals; the average in vivo signal intensity was (1.5 +/- 0.6) x 10(4) radiance (p/sec/cm2/sr); in vivo signal was well above the detection threshold over 3 weeks after injection. In vivo bioluminescent signal is correlated (r2 = 0.8) with the luminometer assay results from homogenized heart samples.
conclusionThe capability of noninvasive imaging of the MLC2v-Fluc in the heart will encourage applications that aim at monitoring and tracking the marker gene expression over time in cells undergoing cardiac differentiation.
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