ArticleMolecular genetics and genomics : MGG2004
Nonsense mutations in the essential gene SUP35 of Saccharomyces cerevisiae are non-lethal.
Article in Molecular genetics and genomics : MGG, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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23 citing papers in PubMed, 52 citations in OpenAlex.
- Deletion Analysis of Phase Separation, Amyloid Formation and Prion Propagation by the Intrinsically Disordered Region of Yeast Sup35 Protein.International journal of molecular sciences · 2026Article
- Prion-Dependent Lethality ofInternational journal of molecular sciences · 2025Article
- Gene Expression Analysis of Yeast Strains with a Nonsense Mutation in the eRF3-Coding Gene Highlights Possible Mechanisms of Adaptation.International journal of molecular sciences · 2024Article
- The [PSIMolecular biology of the cell · 2022Article
- Article
- Structural Bases of Prion Variation in Yeast.International journal of molecular sciences · 2022Review
- Gene Amplification as a Mechanism of Yeast Adaptation to Nonsense Mutations in Release Factor Genes.Genes · 2021Article
- Article
- Nonsense Mutations in the YeastInternational journal of molecular sciences · 2020Article
- Whole genome sequencing data and analyses of the underlyingData in brief · 2019Article
- Design of a New [Frontiers in molecular neuroscience · 2019Article
- Haploid yeast cells undergo a reversible phenotypic switch associated with chromosome II copy number.BMC genetics · 2016Article
- Genome Sequencing and Comparative Analysis of Saccharomyces cerevisiae Strains of the Peterhof Genetic Collection.PloS one · 2016Article
- [PSI(+)] turns 50.Prion · 2015Article
- Effect of charged residues in the N-domain of Sup35 protein on prion [PSI+] stability and propagation.The Journal of biological chemistry · 2013Article
- Regulation of release factor expression using a translational negative feedback loop: a systems analysis.RNA (New York, N.Y.) · 2012Article
- [NSI+] determinant has a pleiotropic phenotypic manifestation that is modulated by SUP35, SUP45, and VTS1 genes.Current genetics · 2012Article
- Unassigned codons, nonsense suppression, and anticodon modifications in the evolution of the genetic code.Journal of molecular evolution · 2011Article
- [NSI (+)]: a novel non-Mendelian nonsense suppressor determinant in Saccharomyces cerevisiae.Current genetics · 2010Article
- The paradox of viable sup45 STOP mutations: a necessary equilibrium between translational readthrough, activity and stability of the protein.Molecular genetics and genomics : MGG · 2009Article
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In the present work we have characterized for the first time non-lethal nonsense mutations in the essential gene SUP35, which codes for the translation termination factor eRF3 in Saccharomyces cerevisiae. The screen used was based on selection for simultaneous suppression of two auxotrophic nonsense mutations. Among 48 mutants obtained, sixteen were distinguished by the production of a reduced amount of eRF3, suggesting the appearance of nonsense mutations. Fifteen of the total mutants were sequenced, and the presence of nonsense mutations was confirmed for nine of them. Thus a substantial fraction of the sup35 mutations recovered are nonsense mutations located in different regions of SUP35, and such mutants are easily identified by the fact that they express reduced amounts of eRF3. Nonsense mutations in the SUP35 gene do not lead to a decrease in levels of SUP35 mRNA and do not influence the steady-state level of eRF1. The ability of these mutations to complement SUP35 gene disruption mutations in different genetic backgrounds and in the absence of any tRNA suppressor mutation was demonstrated. The missense mutations studied, unlike nonsense mutations, do not decrease steady-state amounts of eRF3.
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