ArticleJournal of materials science. Materials in medicine
In vitro reconstructed tissues on hyaluronan-based temporary scaffolding.
Article in Journal of materials science. Materials in medicine. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- PEEK and Hyaluronan-Based 3D Printed Structures: Promising Combination to Improve Bone Regeneration.Molecules (Basel, Switzerland) · 2022Article
- Article
- Stem Cells in Autologous Microfragmented Adipose Tissue: Current Perspectives in Osteoarthritis Disease.International journal of molecular sciences · 2021Review
- Biological Characterization of Human Autologous Pericardium Treated with the Ozaki Procedure for Aortic Valve Reconstruction.Journal of clinical medicine · 2021Article
- Scaffold-free tissue engineering for injured joint surface restoration.Journal of experimental orthopaedics · 2018Review
- Article
- A hyaluronan-based scaffold for the in vitro construction of dental pulp-like tissue.International journal of molecular sciences · 2015Article
- Hyaluronic acid three-dimensional scaffold for surgical revision of retracting scars: a human experimental study.International wound journal · 2013Article
- Matrix-assisted autologous chondrocyte transplantation for remodeling and repair of chondral defects in a rabbit model.Journal of visualized experiments : JoVE · 2013Article
- Active silver nanoparticles for wound healing.International journal of molecular sciences · 2013Article
- Hyaluronan benzyl ester as a scaffold for tissue engineering.International journal of molecular sciences · 2009Review
- Precultivation of engineered human nasal cartilage enhances the mechanical properties relevant for use in facial reconstructive surgery.Annals of surgery · 2006Article
- Gland cell cultures into 3D hyaluronan-based scaffolds.Journal of materials science. Materials in medicine · 2003Article
- Scaffold-free, stem cell-based cartilage repair.Journal of clinical orthopaedics and traumaReview
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Tissue engineering offers the possibility to reconstruct tissue substitutes in order to replace lost or damaged tissues. The availability of appropriate biomaterial devices is essential to allow in vitro cultured cells to behave as in the original tissues in vivo. In our studies we utilized a seminatural biomaterial made up by the benzyl ester of hyaluronan to grow keratinocytes, fibroblasts and chondrocytes. Keratinocytes and fibroblasts were isolated from human foreskin. Cells were separately cultured on two different hyaluronan based biomaterial devices for the first 15 days and then co-cultured for an additional period of 2 weeks. Keratinocytes gave rise to a well-differentiated epithelial layer, while fibroblasts were able to synthesize all the main extracellular molecules inside the biomaterial spaces, forming dermal-like tissues. When these two tissues were co-cultured, a skin equivalent was formed with a dermal-epidermal junction. Chondrocytes were obtained from chick-embryo sterna and cultured for 21 days inside a non-woven scaffolding made up of a hyaluronan-based biomaterial. Cells were able to organize themselves into nodules embedded in a dense metachromatic substance in which type II collagen was present. Data from this study suggest that this novel class of hyaluronan derived biomaterials is suitable for different cell culture and in vitro tissue reconstruction.
Identifiers
15347986What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.