ArticlePlant physiology2004
Two transcription factors are negative regulators of gibberellin response in the HvSPY-signaling pathway in barley aleurone.
Article in Plant physiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 48 citations in OpenAlex.
- SlSEC1- and SlSPY-mediated O-glycosylation stabilizes the transcription factor SlNOR to promote tomato fruit ripening.The Plant cell · 2026Article
- Powdery mildew effectors AVRMolecular plant pathology · 2024Article
- The NAC transcription factors play core roles in flowering and ripening fundamental to fruit yield and quality.Frontiers in plant science · 2023Review
- Novel nucleocytoplasmic protein O-fucosylation by SPINDLY regulates diverse developmental processes in plants.Current opinion in structural biology · 2021Review
- Chemical and Biochemical Strategies To Explore the Substrate Recognition of O-GlcNAc-Cycling Enzymes.Chembiochem : a European journal of chemical biology · 2019Review
- GAFunctional & integrative genomics · 2018Article
- Proteomic analysis reveals O-GlcNAc modification on proteins with key regulatory functions inProceedings of the National Academy of Sciences of the United States of America · 2017Article
- The barley powdery mildew candidate secreted effector protein CSEP0105 inhibits the chaperone activity of a small heat shock protein.Plant physiology · 2015Article
- Differentiation mechanism and function of the cereal aleurone cells and hormone effects on them.Plant cell reports · 2014Review
- Roles of NAC transcription factors in the regulation of biotic and abiotic stress responses in plants.Frontiers in microbiology · 2013Review
- The Arabidopsis O-linked N-acetylglucosamine transferase SPINDLY interacts with class I TCPs to facilitate cytokinin responses in leaves and flowers.The Plant cell · 2012Article
- Characterization of barley (Hordeum vulgare L.) NAC transcription factors suggests conserved functions compared to both monocots and dicots.BMC research notes · 2011Article
- O-GlcNAc protein modification in plants: Evolution and function.Biochimica et biophysica acta · 2010Review
- Identification of genes related to the development of bamboo rhizome bud.Journal of experimental botany · 2010Article
- Functional analysis of HvSPY, a negative regulator of GA response, in barley aleurone cells and Arabidopsis.Planta · 2009Article
- Interactions of two transcriptional repressors and two transcriptional activators in modulating gibberellin signaling in aleurone cells.Plant physiology · 2008Article
- Transcriptional analysis of the sweet orange interaction with the citrus canker pathogens Xanthomonas axonopodis pv. citri and Xanthomonas axonopodis pv. aurantifolii.Molecular plant pathology · 2008Article
- Proteolysis-independent downregulation of DELLA repression in Arabidopsis by the gibberellin receptor GIBBERELLIN INSENSITIVE DWARF1.The Plant cell · 2008Article
- The HvNAC6 transcription factor: a positive regulator of penetration resistance in barley and Arabidopsis.Plant molecular biology · 2007Article
- A comprehensive analysis of the 14-3-3 interactome in barley leaves using a complementary proteomics and two-hybrid approach.Plant physiology · 2007Article
Corrections and comments
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Authors and funding
1 author at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
SPINDLY (SPY) protein from barley (Hordeum vulgare L. cv Himalaya; HvSPY) negatively regulated GA responses in aleurone, and genetic analyses of Arabidopsis thaliana predict that SPY functions in a derepressible GA-signaling pathway. Many, if not all, GA-dependent responses require SPY protein, and to improve our understanding of how the SPY signaling pathway operates, a yeast two-hybrid screen was used to identify both upstream and downstream components that might regulate the activity of the HvSPY protein. A number of proteins from diverse classes were identified using HvSPY as bait and barley cDNA libraries as prey. Two of the HvSPY-interacting (HSI) proteins were transcription factors belonging to the myb and NAC gene families, HSImyb and HSINAC. Interaction occurred via the tetratricopeptide repeat domain of HvSPY and specificity was shown both in vivo and in vitro. Messenger RNAs for these proteins were expressed differentially in many parts of the barley plant but at very low levels. Both HSImyb and HSINAC inhibited the GA(3) up-regulation of alpha-amylase expression in aleurone, both were activators of transcription in yeast, and the green fluorescent protein-HSI fusion proteins were localized in the nucleus. These results are consistent with the model that HSI transcription factors act downstream of HvSPY as negative regulators and that they in turn could activate other negative regulators, forming the HvSPY negative regulator-signaling pathway for GA response.
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