ArticlePlant physiology2004
Cellular and subcellular localization of endogenous nitric oxide in young and senescent pea plants.
Article in Plant physiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 89 papers.
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Who cites it
89 citing papers in PubMed, 381 citations in OpenAlex.
- Peroxisome calcium uptake is dependent on ER-peroxisome membrane contact.Cellular and molecular life sciences : CMLS · 2026Article
- Integration of NO and HPlant communications · 2026Review
- Inhibition of flavohemeproteins enhances the emission and level of nitric oxide in barley root tips.Protoplasma · 2025Article
- Molecular mechanisms of plant productivity enhancement by nano fertilizers for sustainable agriculture.Plant molecular biology · 2024Review
- Characterization of leucine aminopeptidase (LAP) activity in sweet pepper fruits during ripening and its inhibition by nitration and reducing events.Plant cell reports · 2024Article
- Free Radicals Mediated Redox Signaling in Plant Stress Tolerance.Life (Basel, Switzerland) · 2023Review
- Assay of Reactive Oxygen/Nitrogen Species (ROS/RNS) in Arabidopsis Peroxisomes Through Fluorescent Protein Containing a Type 1 Peroxisomal Targeting Signal (PTS1).Methods in molecular biology (Clifton, N.J.) · 2023Article
- Article
- Calcium in peroxisomes: An essential messenger in an essential cell organelle.Frontiers in cell and developmental biology · 2022Review
- Nitric Oxide (NO) Scaffolds the Peroxisomal Protein-Protein Interaction Network in Higher Plants.International journal of molecular sciences · 2021Review
- Crucial Cell Signaling Compounds Crosstalk and Integrative Multi-Omics Techniques for Salinity Stress Tolerance in Plants.Frontiers in plant science · 2021Review
- Multilevel Regulation of Peroxisomal Proteome by Post-Translational Modifications.International journal of molecular sciences · 2019Review
- Gasotransmitters in Action: Nitric Oxide-Ethylene Crosstalk during Plant Growth and Abiotic Stress Responses.Antioxidants (Basel, Switzerland) · 2019Review
- Role of Nitrate Reductase in NO Production in Photosynthetic Eukaryotes.Plants (Basel, Switzerland) · 2019Review
- Impact of Nitric Oxide (NO) on the ROS Metabolism of Peroxisomes.Plants (Basel, Switzerland) · 2019Review
- Nitric Oxide Remodels the Photosynthetic Apparatus upon S-Starvation inPlant physiology · 2019Article
- Nitric Oxide as a Beneficial Signaling Molecule inFrontiers in plant science · 2019Article
- Calcium signaling and salt tolerance are diversely entwined in plants.Plant signaling & behavior · 2019Review
- Chloroplasts at the Crossroad of Photosynthesis, Pathogen Infection and Plant Defense.International journal of molecular sciences · 2018Review
- The peroxisome: an update on mysteries 2.0.Histochemistry and cell biology · 2018Review
29 more citing papers are in PubMed but not listed here.
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Authors and funding
12 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The cellular and subcellular localization of endogenous nitric oxide (NO.) in leaves from young and senescent pea (Pisum sativum) plants was studied. Confocal laser scanning microscopy analysis of pea leaf sections with the fluorescent probe 4,5-diaminofluorescein diacetate revealed that endogenous NO. was mainly present in vascular tissues (xylem and phloem). Green fluorescence spots were also detected in the epidermal cells, palisade and spongy mesophyll cells, and guard cells. In senescent leaves, NO. generation was clearly reduced in the vascular tissues. At the subcellular level, by electron paramagnetic resonance spectroscopy with the spin trap Fe(MGD)(2) and fluorometric analysis with 4,5-diaminofluorescein diacetate, NO. was found to be an endogenous metabolite of peroxisomes. The characteristic three-line electron paramagnetic resonance spectrum of NO., with g = 2.05 and a(N) = 12.8 G, was detected in peroxisomes. By fluorometry, NO. was also found in these organelles, and the level measured of NO. was linearly dependent on the amount of peroxisomal protein. The enzymatic production of NO. from l-Arg (nitric oxide synthase [NOS]-like activity) was measured by ozone chemiluminiscence. The specific activity of peroxisomal NOS was 4.9 nmol NO. mg(-1) protein min(-1); was strictly dependent on NADPH, calmodulin, and BH(4); and required calcium. In senescent pea leaves, the NOS-like activity of peroxisomes was down-regulated by 72%. It is proposed that peroxisomal NO. could be involved in the process of senescence of pea leaves.
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