ArticleThe EMBO journal1992
Palmitoylation alters protein activity: blockade of G(o) stimulation by GAP-43.
Article in The EMBO journal, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 111 citations in OpenAlex.
- The role of protein palmitoylation in disease pathogenesis and therapeutic innovation.Annals of medicine · 2026Review
- GAP-43 and BASP1 in Axon Regeneration: Implications for the Treatment of Neurodegenerative Diseases.Frontiers in cell and developmental biology · 2020Review
- Analysis of IFITM-IFITM Interactions by a Flow Cytometry-Based FRET Assay.International journal of molecular sciences · 2019Article
- Molecular basis of the functions of the mammalian neuronal growth cone revealed using new methods.Proceedings of the Japan Academy. Series B, Physical and biological sciences · 2019Review
- Analysis of the brain palmitoyl-proteome using both acyl-biotin exchange and acyl-resin-assisted capture methods.Scientific reports · 2017Article
- Posttranslational Modifications Regulate the Postsynaptic Localization of PSD-95.Molecular neurobiology · 2017Review
- Distinct palmitoylation events at the amino-terminal conserved cysteines of Env7 direct its stability, localization, and vacuolar fusion regulation in S. cerevisiae.The Journal of biological chemistry · 2014Article
- Palmitoylcarnitine affects localization of growth associated protein GAP-43 in plasma membrane subdomains and its interaction with Gα(o) in neuroblastoma NB-2a cells.Neurochemical research · 2013Article
- Site-specific analysis of protein S-acylation by resin-assisted capture.Journal of lipid research · 2011Article
- Use of micellar electrokinetic chromatography to measure palmitoylation of a peptide.Journal of chromatography. B, Analytical technologies in the biomedical and life sciences · 2008Article
- E2F8 is a nonreceptor activator of heterotrimeric G proteins.Journal of molecular signaling · 2007Article
- Regulation of dendritic branching and filopodia formation in hippocampal neurons by specific acylated protein motifs.Molecular biology of the cell · 2004Article
- Autopalmitoylation of tubulin.Protein science : a publication of the Protein Society · 2000Article
- B-50/GAP-43-induced formation of filopodia depends on Rho-GTPase.Molecular biology of the cell · 1998Article
- Plasma membrane localization is required for RGS4 function in Saccharomyces cerevisiae.Proceedings of the National Academy of Sciences of the United States of America · 1998Article
- Posttranslational modification of tubulin by palmitoylation: I. In vivo and cell-free studies.Molecular biology of the cell · 1997Article
- Exocytosis in single chromaffin cells: regulation by a secretory granule-associated Go protein.Cellular and molecular neurobiology · 1997Article
- GAIP is membrane-anchored by palmitoylation and interacts with the activated (GTP-bound) form of G alpha i subunits.Proceedings of the National Academy of Sciences of the United States of America · 1996Article
- Human palmitoyl protein thioesterase: evidence for lysosomal targeting of the enzyme and disturbed cellular routing in infantile neuronal ceroid lipofuscinosis.The EMBO journal · 1996Article
- Cytoplasmic tail length influences fatty acid selection for acylation of viral glycoproteins.The Biochemical journal · 1996Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The addition of palmitate to cysteine residues enhances the hydrophobicity of proteins, and consequently their membrane association. Here we have investigated whether this type of fatty acylation also regulates protein-protein interactions. GAP-43 is a neuronal protein that increases guanine nucleotide exchange by heterotrimeric G proteins. Two cysteine residues near the N-terminus of GAP-43 are subject to palmitoylation, and are necessary for membrane binding as well as for G(o) activation. N-terminal peptides, which include these cysteines, stimulate G(o). Monopalmitoylation reduces, and dipalmitoylation abolishes the activity of the peptides. The activity of GAP-43 protein purified from brain also is reversibly blocked by palmitoylation. This suggests that palmitoylation controls a cycle of GAP-43 between an acylated, membrane-bound reservoir of inactive GAP-43, and a depalmitoylated, active pool of protein.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.