ArticleBiophysical journal1992
Effects of protein concentration on IgE receptor mobility in rat basophilic leukemia cell plasma membranes.
Article in Biophysical journal, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 33 citations in OpenAlex.
- Article
- Watt W. Webb: His measurements of the seemingly inaccessible broadened the horizons of biophysics.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Imaging FCS delineates subtle heterogeneity in plasma membranes of resting mast cells.Molecular biology of the cell · 2020Article
- Article
- Imaging Membrane Curvature inside a FcεRI-Centric Synapse in RBL-2H3 Cells Using TIRF Microscopy with Polarized Excitation.Journal of imaging · 2019Article
- Article
- Single-particle tracking of immunoglobulin E receptors (FcεRI) in micron-sized clusters and receptor patches.FEBS letters · 2012Article
- Spatio-temporal signaling in mast cells.Advances in experimental medicine and biology · 2011Review
- Distribution and dynamics of rat basophilic leukemia immunoglobulin E receptors (FcepsilonRI) on planar ligand-presenting surfaces.Biophysical journal · 2010Article
- Actin restricts FcepsilonRI diffusion and facilitates antigen-induced receptor immobilization.Nature cell biology · 2008Article
- Long-range nonanomalous diffusion of quantum dot-labeled aquaporin-1 water channels in the cell plasma membrane.Biophysical journal · 2008Article
- Large-scale fluid/fluid phase separation of proteins and lipids in giant plasma membrane vesicles.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- Effect of hydrodynamic interactions on the diffusion of integral membrane proteins: diffusion in plasma membranes.Biophysical journal · 1995Article
- Lateral diffusion in an archipelago. Single-particle diffusion.Biophysical journal · 1993Article
- Cell shape-dependent rectification of surface receptor transport in a sinusoidal electric field.Biophysical journal · 1993Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The ability of variations of membrane protein concentrations to modulate the lateral diffusion rate of an exemplary membrane protein has been studied in healthy and osmotically shocked cultured cells of the rat basophilic leukemia cell line, 2H3 subclone. Cell surface protein was redistributed by the method of in situ electrophoresis; exposure to electric fields of 1.25-5 V/cm results in cathodal migration of the majority of the surface proteins on this cell type (Ryan, T. A., J. Myers, D. Holowka, B. Baird, and W. W. Webb. Science [Wash. DC]. 239:61-64). Even in these small fields, the steady-state distribution becomes "crowded" with more than an 80% protein occupancy of accessible membrane area at the cathodal end of these spheroidal cells, and the anodal end becomes significantly depleted. We have employed fringe pattern fluorescence photobleaching with CCD imaging detection to measure lateral diffusion coefficients of the liganded IgE receptor on both crowded and uncrowded regions of individual rat basophilic leukemia cells. We find no significant difference in lateral diffusion rates in these regions. Cells swollen by hypoosmotic stress exhibit faster diffusion overall, with the uncrowded regions having a significantly greater increase in diffusion coefficient than the crowded regions. These results are consistent with the partial or total release of cytoskeletal constraints to membrane protein diffusion induced by osmotic stress.
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