ArticleBiophysical journal2004
Pseudopod projection and cell spreading of passive leukocytes in response to fluid shear stress.
Article in Biophysical journal, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed.
- Piezo1 expression in neutrophils regulates shear-induced NETosis.Nature communications · 2024Article
- Go with the flow: GEF-H1 mediated shear stress mechanotransduction in neutrophils.Small GTPases · 2020Review
- Effects of Transient Exposure to High Shear on Neutrophil Rolling Behavior.Cellular and molecular bioengineering · 2018Article
- Hydrodynamics in Cell Studies.Chemical reviews · 2018Review
- Fluid shear-induced cathepsin B release in the control of Mac1-dependent neutrophil adhesion.Journal of leukocyte biology · 2017Article
- GEF-H1 is necessary for neutrophil shear stress-induced migration during inflammation.The Journal of cell biology · 2016Article
- Effect of Pseudopod Extensions on Neutrophil Hemodynamic Transport Near a Wall.Cellular and molecular bioengineering · 2016Article
- Modeling the Mechanosensitivity of Neutrophils Passing through a Narrow Channel.Biophysical journal · 2015Article
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- T lymphocytes orient against the direction of fluid flow during LFA-1-mediated migration.Biophysical journal · 2013Article
- Forces on a wall-bound leukocyte in a small vessel due to red cells in the blood stream.Biophysical journal · 2012Article
- Shear-induced resistance to neutrophil activation via the formyl peptide receptor.Biophysical journal · 2012Article
- Actin polymerization stabilizes α4β1 integrin anchors that mediate monocyte adhesion.The Journal of cell biology · 2012Article
- Internalization of Formyl Peptide Receptor in Leukocytes Subject to Fluid Stresses.Cellular and molecular bioengineering · 2010Article
- Critical stresses for cancer cell detachment in microchannels.European biophysics journal : EBJ · 2009Article
- Vav1 is essential for mechanotactic crawling and migration of neutrophils out of the inflamed microvasculature.Journal of immunology (Baltimore, Md. : 1950) · 2009Article
- Modeling cell interactions under flow.Journal of mathematical biology · 2009Review
- Mechanical forces induced by the transendothelial migration of human neutrophils.Biophysical journal · 2008Article
- Recoil and stiffening by adherent leukocytes in response to fluid shear.Biophysical journal · 2008Article
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
Recent evidence suggests that circulating leukocytes respond to physiological levels of fluid shear stress. This study was designed to examine the shear stress response of individual leukocytes adhering passively to a glass surface. Human leukocytes were exposed to a step fluid shear stress with amplitude between 0.2 and 4 dyn/cm(2) and duration between 1 and 20 min. The response of the cells was determined in the form of projected cell area measurements by high-resolution observation before, during, and after fluid shear application. All cells selected initially had a round morphology. After application of fluid shear many cells projected pseudopodia and spread on the glass surface. The number of leukocytes responding with pseudopod projection and the extent of cell spreading increased with increasing amplitude and duration of fluid shear stress. Pseudopod projection after exposure to a step fluid shear occurs following a delay that is insensitive to the shear stress amplitude and duration. Leukocytes that did not project pseudopodia and spread in response to low shear stress could be shown to respond to a second shear step of higher amplitude. The spreading response requires an intact actin network and activated myosin molecules. Depleting the cell glycocalyx with protease treatment enhances the spreading response in sheared leukocytes. These results indicate that passive leukocytes respond to fluid shear stress with active pseudopod projection and cell spreading. This behavior may contribute to cell spreading on endothelium and other cells as well as to transendothelial migration of leukocytes in the microcirculation.
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Registered trials
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