ArticleBiophysical journal2004
Micro-volume couette flow sample orientation for absorbance and fluorescence linear dichroism.
Article in Biophysical journal, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Couette Flow Linear Dichroism Spectroscopy.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Flow dichroism of DNA can be quantitatively predicted via coarse-grained molecular simulations.Biophysical journal · 2024Article
- Linear Dichroism Measurements for the Study of Protein-DNA Interactions.International journal of molecular sciences · 2023Review
- A Cytotoxic Bis(1,2,3-triazol-5-ylidene)carbazolide Gold(III) Complex Targets DNA by Partial Intercalation.Chemistry (Weinheim an der Bergstrasse, Germany) · 2021Article
- Insight into the Mechanism of Action and Peptide-Membrane Interactions of Aib-Rich Peptides: Multitechnique Experimental and Theoretical Analysis.Chembiochem : a European journal of chemical biology · 2021Article
- Flow Linear Dichroism of Protein-Membrane Systems.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Light scattering corrections to linear dichroism spectroscopy for liposomes in shear flow using calcein fluorescence and modified Rayleigh-Gans-Debye-Mie scattering.Biophysical reviews · 2018Review
- Linear dichroism of visible-region chromophores using M13 bacteriophage as an alignment scaffold.RSC advances · 2018Article
- Intrinsic protein disorder could be overlooked in cocrystallization conditions: An SRCD case study.Protein science : a publication of the Protein Society · 2016Article
- Substrate binding activates the designed triple mutant of the colicin E7 metallonuclease.Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry · 2014Article
- Fine tuning of the catalytic activity of colicin E7 nuclease domain by systematic N-terminal mutations.Protein science : a publication of the Protein Society · 2014Article
- Is photocleavage of DNA by YOYO-1 using a synchrotron radiation light source sequence dependent?European biophysics journal : EBJ · 2011Article
- Is DNA a worm-like chain in Couette flow? In search of persistence length, a critical review.Science progress · 2009Review
- Breaking the 200 nm limit for routine flow linear dichroism measurements using UV synchrotron radiation.Biophysical journal · 2008Article
- In vitro dimerization of the bovine papillomavirus E5 protein transmembrane domain.Biochemistry · 2008Article
- How to study DNA and proteins by linear dichroism spectroscopy.Science progress · 2008Review
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Linear dichroism (LD) can be used to study the alignment of absorbing chromophores within long molecules. In particular, Couette flow LD has been used to good effect in probing ligand binding to DNA and to fibrous proteins. This technique has been previously limited by large sample requirements. Here we report the design and application of a new micro-volume Couette flow cell that significantly enhances the potential applications of flow LD spectroscopy by reducing the sample requirements for flow linear dichroism to 25 microL (with concentrations such that the absorbance maximum of the sample in a 1-cm pathlength cuvette is approximately 1). The micro-volume Couette cell has also enabled the measurement of fluorescence-detected Couette flow linear dichroism. This new technique enables the orientation of fluorescent ligands to be probed even when their electronic transitions overlap with those of the macromolecule and conversely. The potential of flow-oriented fluorescence dichroism and application of the micro-volume Couette LD cell are illustrated by the collection of data for DNA with minor groove and intercalating ligands: DAPI, Hoechst, and ethidium bromide. As with conventional fluorescence, improved sensitivity compared with absorbance LD is to be expected after instrumentation optimization.
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