ArticleBiophysical journal2004
Organization of Ca2+ release units in excitable smooth muscle of the guinea-pig urinary bladder.
Article in Biophysical journal, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed.
- Endoplasmic Reticulum-Plasma Membrane Junctions as Sites of Depolarization-Induced CaAnnual review of physiology · 2023Review
- Junctophilins: Key Membrane Tethers in Muscles and Neurons.Frontiers in molecular neuroscience · 2021Review
- Calsequestrin: a well-known but curious protein in skeletal muscle.Experimental & molecular medicine · 2020Review
- On a Magical Mystery Tour with 8-Bromo-Cyclic ADP-Ribose: From All-or-None Block to Nanojunctions and the Cell-Wide Web.Molecules (Basel, Switzerland) · 2020Review
- Preparation and Utilization of Freshly Isolated Human Detrusor Smooth Muscle Cells for Characterization of 9-Phenanthrol-Sensitive Cation Currents.Journal of visualized experiments : JoVE · 2020Article
- Elementary calcium signaling in arterial smooth muscle.Channels (Austin, Tex.) · 2019Review
- Membrane potential and Ca2+ concentration dependence on pressure and vasoactive agents in arterial smooth muscle: A model.The Journal of general physiology · 2015Article
- Facilitated hyperpolarization signaling in vascular smooth muscle-overexpressing TRIC-A channels.The Journal of biological chemistry · 2013Article
- CaV1.2 sparklets in heart and vascular smooth muscle.Journal of molecular and cellular cardiology · 2013Review
- Local regulation of L-type Ca²⁺ channel sparklets in arterial smooth muscle.Microcirculation (New York, N.Y. : 1994) · 2013Review
- Pan-junctional sarcoplasmic reticulum in vascular smooth muscle: nanospace Ca2+ transport for site- and function-specific Ca2+ signalling.The Journal of physiology · 2013Review
- Structure and dynamics of the actin-based smooth muscle contractile and cytoskeletal apparatus.Journal of muscle research and cell motility · 2012Review
- Subplasma membrane Ca2+ signals.IUBMB life · 2012Review
- Ca2+ entry following P2X receptor activation induces IP3 receptor-mediated Ca2+ release in myocytes from small renal arteries.British journal of pharmacology · 2011Article
- STIM proteins and the endoplasmic reticulum-plasma membrane junctions.Annual review of biochemistry · 2011Review
- Natural inequalities: why some L-type Ca2+ channels work harder than others.The Journal of general physiology · 2010Article
- Proximity of Na+ -Ca2+ -exchanger and sarco/endoplasmic reticulum Ca2+ pump in pig coronary artery smooth muscle: fluorescence microscopy.Molecular and cellular biochemistry · 2010Article
- Molecular and biophysical mechanisms of Ca2+ sparklets in smooth muscle.Journal of molecular and cellular cardiology · 2009Review
- Three-dimensional electron microscopic reconstruction of intracellular organellar arrangements in vascular smooth muscle--further evidence of nanospaces and contacts.Journal of cellular and molecular medicine · 2009Article
- A univariate model of calcium release in the dyadic cleft of cardiac myocytes.Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference · 2009Article
Corrections and comments
- Erratum issued
Authors and funding
7 authors.
Funding
Abstract
Ca(2+) release from internal stores (sarcoplasmic reticulum or SR) in smooth muscles is initiated either via pharmaco-mechanical coupling due to the action of an agonist and involving IP3 receptors, or via excitation-contraction coupling, mostly involving L-type calcium channels in the plasmalemma (DHPRs), and ryanodine receptors (RyRs), or Ca(2+) release channels of the SR. This work focuses attention on the structural basis for the coupling between DHPRs and RyRs in phasic smooth muscle cells of the guinea-pig urinary bladder. Immunolabeling shows that two proteins of the SR: calsequestrin and the RyR, and one protein the plasmalemma, the L-type channel or DHPR, are colocalized with each other within numerous, peripherally located sites located within the caveolar domains. Electron microscopy images from thin sections and freeze-fracture replicas identify feet in small peripherally located SR vesicles containing calsequestrin and distinctive large particles clustered within small membrane areas. Both feet and particle clusters are located within caveolar domains. Correspondence between the location of feet and particle clusters and of RyR- and DHPR-positive foci allows the conclusion that calsequestrin, RyRs, and L-type Ca(2+) channels are associated with peripheral couplings, or Ca(2+) release units, constituting the key machinery involved in excitation-contraction coupling. Structural analogies between smooth and cardiac muscle excitation-contraction coupling complexes suggest a common basic mechanism of action.
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