ArticleApplied and environmental microbiology2004
Raw cow milk bacterial population shifts attributable to refrigeration.
Article in Applied and environmental microbiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
29 citing papers in PubMed.
- The Impact of Cold Storage and Seasonality on Raw Cow Milk Microbiota, Assessed by Conventional and Viability PCR.Animals : an open access journal from MDPI · 2026Article
- Techniques to Detect and Quantify the Bacterial Metalloprotease AprX in Bovine Milk: A Review.Comprehensive reviews in food science and food safety · 2026Review
- The Effects of Milking and Cleaning Procedures on the Quality and Microbiome of Raw Goat Milk.Foods (Basel, Switzerland) · 2025Article
- Article
- Screening of lactic acid bacteria from the milk of Sahiwal cows and characterization of their probiotic potential for preventing bovine mastitis.International microbiology : the official journal of the Spanish Society for Microbiology · 2025Article
- Biofilm formation by heat-resistant dairy bacteria: multispecies biofilm model under static and dynamic conditions.Applied and environmental microbiology · 2023Article
- Prevalence, Virulence, and Antibiotics Gene Profiles inMicroorganisms · 2023Article
- New insights into the raw milk microbiota diversity from animals with a different genetic predisposition for feed efficiency and resilience to mastitis.Scientific reports · 2022Article
- Bacterial Complexity of Traditional Mountain Butter Is Affected by the Malga-Farm of Production.Microorganisms · 2021Article
- Simultaneous quantification of the most common and proteolytic Pseudomonas species in raw milk by multiplex qPCR.Applied microbiology and biotechnology · 2021Article
- Multifaceted effects of milk-exosomes (Mi-Exo) as a modulator of scar-free wound healing.Nanoscale advances · 2021Article
- Spoilage Lactic Acid Bacteria in the Brewing Industry.Journal of microbiology and biotechnology · 2020Review
- Impact of yeast and lactic acid bacteria on mastitis and milk microbiota composition of dairy cows.AMB Express · 2020Article
- Article
- Characterization ofFrontiers in microbiology · 2017Article
- The Biodiversity of the Microbiota Producing Heat-Resistant Enzymes Responsible for Spoilage in Processed Bovine Milk and Dairy Products.Frontiers in microbiology · 2017Review
- NFrontiers in microbiology · 2017Article
- A Novel Lactobacilli-Based Teat Disinfectant for Improving Bacterial Communities in the Milks of Cow Teats with Subclinical Mastitis.Frontiers in microbiology · 2017Article
- Mesophilic Lactic Acid Bacteria Diversity Encountered in Brazilian Farms Producing Milk with Particular Interest in Lactococcus lactis Strains.Current microbiology · 2016Article
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We monitored the dynamic changes in the bacterial population in milk associated with refrigeration. Direct analyses of DNA by using temporal temperature gel electrophoresis (TTGE) and denaturing gradient gel electrophoresis (DGGE) allowed us to make accurate species assignments for bacteria with low-GC-content (low-GC%) (<55%) and medium- or high-GC% (>55%) genomes, respectively. We examined raw milk samples before and after 24-h conservation at 4 degrees C. Bacterial identification was facilitated by comparison with an extensive bacterial reference database ( approximately 150 species) that we established with DNA fragments of pure bacterial strains. Cloning and sequencing of fragments missing from the database were used to achieve complete species identification. Considerable evolution of bacterial populations occurred during conservation at 4 degrees C. TTGE and DGGE are shown to be a powerful tool for identifying the main bacterial species of the raw milk samples and for monitoring changes in bacterial populations during conservation at 4 degrees C. The emergence of psychrotrophic bacteria such as Listeria spp. or Aeromonas hydrophila is demonstrated.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.