ArticleApplied and environmental microbiology2004
Characterization of a highly enriched dehalococcoides-containing culture that grows on vinyl chloride and trichloroethene.
Article in Applied and environmental microbiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 55 papers.
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Who cites it
55 citing papers in PubMed.
- Environmental considerations and bioremediation applications of the metal-, nitrate-, and organohalide-respiring bacteriumApplied and environmental microbiology · 2026Review
- Dechlorination of mixed chlorinated organophosphate esters (V6 and TCEP) and associated reductive dehalogenase gene expression byApplied and environmental microbiology · 2026Article
- Unveiling organohalide respiration potential in River Nile sediments via 16S rRNA gene amplicon sequencing of endogenous bacterial communities.BMC microbiology · 2025Article
- Developing a microbial community structure index (MCSI) as an approach to evaluate and optimize bioremediation performance.Biodegradation · 2024Article
- Salinity determines performance, functional populations, and microbial ecology in consortia attenuating organohalide pollutants.The ISME journal · 2023Article
- Long-term survival ofFEMS microbes · 2022Article
- Identification of Reductive Dehalogenases That Mediate Complete Debromination of Penta- and Tetrabrominated Diphenyl Ethers inApplied and environmental microbiology · 2021Article
- Growth of Dehalococcoides spp. and increased abundance of reductive dehalogenase genes in anaerobic PCB-contaminated sediment microcosms.Environmental science and pollution research international · 2020Article
- Normalized Quantitative PCR Measurements as Predictors for Ethene Formation at Sites Impacted with Chlorinated Ethenes.Environmental science & technology · 2018Article
- Biomarkers' Responses to Reductive Dechlorination Rates and Oxygen Stress in Bioaugmentation Culture KB-1Microorganisms · 2018Article
- Chlorinated Electron Acceptor Abundance Drives Selection ofFrontiers in microbiology · 2018Article
- Widespread Distribution of Dehalococcoides mccartyi in the Houston Ship Channel and Galveston Bay, Texas, Sediments and the Potential for Reductive Dechlorination of PCDD/F in an Estuarine Environment.Marine biotechnology (New York, N.Y.) · 2016Article
- Reductive dechlorination in recalcitrant sources of chloroethenes in the transition zone between aquifers and aquitards.Environmental science and pollution research international · 2016Article
- Loop-Mediated Isothermal Amplification (LAMP) for Rapid Detection and Quantification of Dehalococcoides Biomarker Genes in Commercial Reductive Dechlorinating Cultures KB-1 and SDC-9.Applied and environmental microbiology · 2016Article
- Article
- Identification of specific corrinoids reveals corrinoid modification in dechlorinating microbial communities.Environmental microbiology · 2015Article
- The roles of methanogens and acetogens in dechlorination of trichloroethene using different electron donors.Environmental science and pollution research international · 2015Article
- Impact of a wastewater treatment plant on microbial community composition and function in a hyporheic zone of a eutrophic river.Scientific reports · 2015Article
- Identity and Substrate Specificity of Reductive Dehalogenases Expressed in Dehalococcoides-Containing Enrichment Cultures Maintained on Different Chlorinated Ethenes.Applied and environmental microbiology · 2015Article
- Enumeration of Organohalide Respirers in Municipal Wastewater Anaerobic Digesters.Microbiology insights · 2015Article
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3 authors.
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Abstract
A highly enriched culture that reductively dechlorinates trichloroethene (TCE), cis-1,2-dichloroethene (cDCE), and vinyl chloride (VC) to ethene without methanogenesis is described. The Dehalococcoides strain in this enrichment culture had a yield of (5.6 +/- 1.4) x 10(8) 16S rRNA gene copies/micromol of Cl(-) when grown on VC and hydrogen. Unlike the other VC-degrading cultures described in the literature, strains VS and BAV1, this culture maintained the ability to grow on TCE with a yield of (3.6 +/- 1.3) x 10(8) 16S rRNA gene copies/micromol of Cl(-). The yields on an electron-equivalent basis measured for the culture grown on TCE and on VC were not significantly different, indicating that both substrates supported growth equally well. PCR followed by denaturing gradient gel electrophoresis, cloning, and phylogenetic analyses revealed that this culture contained one Dehalococcoides 16S rRNA gene sequence, designated KB-1/VC, that was identical (over 1,386 bp) to the sequences of previously described organisms FL2 and CBDB1. A second Dehalococcoides sequence found in separate KB-1 enrichment cultures maintained on cDCE, TCE, and tetrachloroethene was no longer present in the VC-H(2) enrichment culture. This second Dehalococcoides sequence was identical to that of BAV1. As neither FL2 nor CBDB1 can dechlorinate VC to ethene in a growth-related fashion, it is clear that current 16S rRNA gene-based analyses do not provide sufficient information to distinguish between metabolically diverse members of the Dehalococcoides group.
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