ArticleApplied and environmental microbiology2004
Role of hexose transport in control of glycolytic flux in Saccharomyces cerevisiae.
Article in Applied and environmental microbiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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Who cites it
43 citing papers in PubMed.
- The return of metabolism: biochemistry and physiology of glycolysis.Biological reviews of the Cambridge Philosophical Society · 2026Review
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- Overflow metabolism in bacterial, yeast, and mammalian cells: different names, same game.Molecular systems biology · 2025Review
- Quantifying intracellular glucose levels when yeast is grown in glucose media.Scientific reports · 2023Article
- Microbial population dynamics decouple growth response from environmental nutrient concentration.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Rewiring regulation on respiro-fermentative metabolism relieved Crabtree effects inSynthetic and systems biotechnology · 2022Article
- Yeast as a tool for membrane protein production and structure determination.FEMS yeast research · 2022Article
- Consideration of Maintenance in Wine Fermentation Modeling.Foods (Basel, Switzerland) · 2022Article
- Improving recombinant protein production by yeast through genome-scale modeling using proteome constraints.Nature communications · 2022Article
- Activation of cryptic xylose metabolism by a transcriptional activator Znf1 boosts up xylitol production in the engineered Saccharomyces cerevisiae lacking xylose suppressor BUD21 gene.Microbial cell factories · 2022Article
- What makes Komagataella phaffii non-conventional?FEMS yeast research · 2021Review
- Adaptations in metabolism and protein translation give rise to the Crabtree effect in yeast.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Pentafluoro-3-hydroxy-pent-2-en-1-ones Potently Inhibit FNT-Type Lactate Transporters from all Five Human-Pathogenic Plasmodium Species.ChemMedChem · 2021Article
- Predicting microbial growth dynamics in response to nutrient availability.PLoS computational biology · 2021Article
- A Putative Bet-Hedging Strategy Buffers Budding Yeast against Environmental Instability.Current biology : CB · 2020Article
- Article
- Novel insights into the organic solute transporter alpha/beta, OSTα/β: From the bench to the bedside.Pharmacology & therapeutics · 2020Review
- Measuring glycolytic flux in single yeast cells with an orthogonal synthetic biosensor.Molecular systems biology · 2019Article
- Differential scaling between G1 protein production and cell size dynamics promotes commitment to the cell division cycle in budding yeast.Nature cell biology · 2019Article
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The yeast Saccharomyces cerevisiae predominantly ferments glucose to ethanol at high external glucose concentrations, irrespective of the presence of oxygen. In contrast, at low external glucose concentrations and in the presence of oxygen, as in a glucose-limited chemostat, no ethanol is produced. The importance of the external glucose concentration suggests a central role for the affinity and maximal transport rates of yeast's glucose transporters in the control of ethanol production. Here we present a series of strains producing functional chimeras between the hexose transporters Hxt1 and Hxt7, each of which has distinct glucose transport characteristics. The strains display a range of decreasing glycolytic rates resulting in a proportional decrease in ethanol production. Using these strains, we show for the first time that at high glucose levels, the glucose uptake capacity of wild-type S. cerevisiae does not control glycolytic flux during exponential batch growth. In contrast, our chimeric Hxt transporters control the rate of glycolysis to a high degree. Strains whose glucose uptake is mediated by these chimeric transporters will undoubtedly provide a powerful tool with which to examine in detail the mechanism underlying the switch between fermentation and respiration in S. cerevisiae and will provide new tools for the control of industrial fermentations.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.