ArticleApplied and environmental microbiology2004
Comparative proteomic analysis of extracellular proteins of enterohemorrhagic and enteropathogenic Escherichia coli strains and their ihf and ler mutants.
Article in Applied and environmental microbiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 65 citations in OpenAlex.
- Substrate secretion by different EHEC secretion systems during their interaction with epithelial cells.Virulence · 2026Article
- The role of integration host factor in biofilm and virulence of high-alcohol-producingMicrobiology spectrum · 2023Article
- Characterization of Molecular Chaperone GroEL as a Potential Virulence Factor inFoods (Basel, Switzerland) · 2023Article
- GrlR, a negative regulator in enteropathogenicFrontiers in microbiology · 2023Article
- Environment Controls LEE Regulation in EnteropathogenicFrontiers in microbiology · 2018Review
- Blood Serum Affects Polysaccharide Production and Surface Protein Expression inAdvances in biotechnology & microbiology (Newbury, Calif.) · 2017Article
- Characterization of the universal stress protein F from atypical enteropathogenic Escherichia coli and its prevalence in Enterobacteriaceae.Protein science : a publication of the Protein Society · 2016Article
- Excreted Cytoplasmic Proteins Contribute to Pathogenicity in Staphylococcus aureus.Infection and immunity · 2016Article
- Excretion of cytoplasmic proteins in Staphylococcus is most likely not due to cell lysis.Current genetics · 2016Review
- Expression Regulation of Polycistronic lee3 Genes of Enterohaemorrhagic Escherichia coli.PloS one · 2016Article
- Common Non-classically Secreted Bacterial Proteins with Experimental Evidence.Current microbiology · 2016Review
- DNABII proteins play a central role in UPEC biofilm structure.Molecular microbiology · 2015Article
- Secretome analysis revealed adaptive and non-adaptive responses of the Staphylococcus carnosus femB mutant.Proteomics · 2015Article
- DNA looping-dependent autorepression of LEE1 P1 promoters by Ler in enteropathogenic Escherichia coli (EPEC).Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Proteomic View of Interactions of Shiga Toxin-Producing Escherichia coli with the Intestinal Environment in Gnotobiotic Piglets.PloS one · 2013Article
- Correlating levels of type III secretion and secreted proteins with fecal shedding of Escherichia coli O157:H7 in cattle.Infection and immunity · 2012Article
- Proteomic analysis of Bacillus thuringiensis strain 4.0718 at different growth phases.TheScientificWorldJournal · 2012Article
- Aberrant community architecture and attenuated persistence of uropathogenic Escherichia coli in the absence of individual IHF subunits.PloS one · 2012Article
- Enterohemorrhagic Escherichia coli induce attaching and effacing lesions and hemorrhagic colitis in human and bovine intestinal xenograft models.Disease models & mechanisms · 2011Article
- Characterizing the Escherichia coli O157:H7 proteome including protein associations with higher order assemblies.PloS one · 2011Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Enterohemorrhagic and enteropathogenic Escherichia coli (EHEC and EPEC, respectively) strains are closely related human pathogens that are responsible for food-borne epidemics in many countries. Integration host factor (IHF) and the locus of enterocyte effacement-encoded regulator (Ler) are needed for the expression of virulence genes in EHEC and EPEC, including the elicitation of actin rearrangements for attaching and effacing lesions. We applied a proteomic approach, using two-dimensional polyacrylamide gel electrophoresis in combination with matrix-assisted laser desorption ionization-time of flight mass spectrometry and a protein database search, to analyze the extracellular protein profiles of EHEC EDL933, EPEC E2348/69, and their ihf and ler mutants. Fifty-nine major protein spots from the extracellular proteomes were identified, including six proteins of unknown function. Twenty-six of them were conserved between EHEC EDL933 and EPEC E2348/69, while some of them were strain-specific proteins. Four common extracellular proteins (EspA, EspB, EspD, and Tir) were regulated by both IHF and Ler in EHEC EDL933 and EPEC E2348/69. TagA in EHEC EDL933 and EspC and EspF in EPEC E2348/69 were present in the wild-type strains but absent from their respective ler and ihf mutants, while FliC was overexpressed in the ihf mutant of EPEC E2348/69. Two dominant forms of EspB were found in EHEC EDL933 and EPEC E2348/69, but the significance of this is unknown. These results show that proteomics is a powerful platform technology for accelerating the understanding of EPEC and EHEC pathogenesis and identifying markers for laboratory diagnoses of these pathogens.
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