Evidence map›Paper›PMID 15345399›Full record

ArticleApplied and environmental microbiology2004

Cell density-dependent gene contributes to efficient seed colonization by Pseudomonas putida KT2440.

Manuel Espinosa-Urgel, Juan-Luis Ramos

Open access · greenAbstract read
In one paragraph

Article in Applied and environmental microbiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.

0numbers the graph read from it
0cells of the map it votes in
25citing papers in PubMed
1.0field-weighted citation impact, top 28% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

25 citing papers in PubMed, 71 citations in OpenAlex.

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  12. Identification of a Chemoreceptor for C2 and C3 Carboxylic Acids.Applied and environmental microbiology · 2015
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 1 institution in 1 country.

Manuel Espinosa-UrgelDepartment of Plant Biochemistry and Molecular and Cellular Biology, Estacíon Experimental del Zaidín, CSIC, Granada 18008, Spain. manuel.espinosa@eez.csic.es
Juan-Luis Ramos
Estación Experimental del Zaidín · ES

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

We have characterized the expression pattern of a gene, ddcA, involved in initial colonization of corn seeds by Pseudomonas putida KT2440. The ddcA gene codes for a putative membrane polypeptide belonging to a family of conserved proteins of unknown function. Members of this family are widespread among prokaryotes and include the products of a Salmonella enterica serovar Typhimurium gene expressed during invasion of macrophages and psiE, an Escherichia coli phosphate starvation-inducible gene. Although its specific role is undetermined, the presence of ddcA in multicopy restored the seed adhesion capacity of a KT2440 ddcA mutant. Expression of ddcA is growth phase regulated, being maximal at the beginning of stationary phase. It is independent of RpoS, nutrient depletion, or phosphate starvation, and it is not the result of changes in the medium pH during growth. Expression of ddcA is directly dependent on cell density, being also stimulated by the addition of conditioned medium and of seed exudates. This is the first evidence suggesting the existence of a quorum-sensing system in P. putida KT2440. The potential implication of such a signaling process in seed adhesion and colonization by the bacterium is discussed.

Indexed as

Amino Acid SequenceBacterial ProteinsBase SequenceDNA PrimersGenes, BacterialKineticsMolecular Sequence DataPolymerase Chain ReactionPseudomonas putidaSequence AlignmentSequence Homology, Amino AcidBacterial ProteinsDNA Primers

Identifiers

PMID15345399
PMCPMC520864
OpenAlexW2014202830

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.