ArticleApplied and environmental microbiology2004
Characterization and heterologous expression of the oxalyl coenzyme A decarboxylase gene from Bifidobacterium lactis.
Article in Applied and environmental microbiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 72 citations in OpenAlex.
- Predicting probiotic success: lessons from Oxalobacter and oxalate metabolism.NPJ biofilms and microbiomes · 2026Review
- Gut and Urinary Microbiota in Cats with Kidney Stones.Microorganisms · 2024Article
- Therapeutic effects of probiotics and herbal medications on oxalate nephrolithiasis: a mini systematic review.Iranian journal of microbiology · 2024Review
- The urinary microbiota composition and functionality of calcium oxalate stone formers.Frontiers in cellular and infection microbiology · 2024Article
- Oxalate and oxalotrophy: an environmental perspective.Sustainable microbiology · 2024Review
- Probiotic Oxalate-Degrading Bacteria: New Insight of Environmental Variables and Expression of the oxc and frc Genes on Oxalate Degradation Activity.Foods (Basel, Switzerland) · 2022Review
- Review
- Article
- High-quality whole-genome sequence analysis of Lactobacillus paragasseri UBLG-36 reveals oxalate-degrading potential of the strain.PloS one · 2021Article
- Abundance, Functional, and Evolutionary Analysis of Oxalyl-Coenzyme A Decarboxylase in Human Microbiota.Frontiers in microbiology · 2020Article
- Bifidobacterium animalis subsp. lactis decreases urinary oxalate excretion in a mouse model of primary hyperoxaluria.Urolithiasis · 2015Article
- Glyphosate, pathways to modern diseases III: Manganese, neurological diseases, and associated pathologies.Surgical neurology international · 2015Article
- Screening of indigenous oxalate degrading lactic acid bacteria from human faeces and South Indian fermented foods: assessment of probiotic potential.TheScientificWorldJournal · 2014Article
- Acute probiotic ingestion reduces gastrointestinal oxalate absorption in healthy subjects.Urological research · 2012Article
- Factors related to colonization with Oxalobacter formigenes in U.S. adults.Journal of endourology · 2011Article
- Oxalate-degrading activity in Bifidobacterium animalis subsp. lactis: impact of acidic conditions on the transcriptional levels of the oxalyl coenzyme A (CoA) decarboxylase and formyl-CoA transferase genes.Applied and environmental microbiology · 2010Article
- Probiotic-induced reduction of gastrointestinal oxalate absorption in healthy subjects.Urological research · 2010Article
- Comparison of the complete genome sequences of Bifidobacterium animalis subsp. lactis DSM 10140 and Bl-04.Journal of bacteriology · 2009Article
- Analysis of the genome sequence of Lactobacillus gasseri ATCC 33323 reveals the molecular basis of an autochthonous intestinal organism.Applied and environmental microbiology · 2008Article
- Adaptation and response of Bifidobacterium animalis subsp. lactis to bile: a proteomic and physiological approach.Applied and environmental microbiology · 2007Article
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Oxalyl coenzyme A (CoA) decarboxylase (Oxc) is a key enzyme in the catabolism of the highly toxic compound oxalate, catalyzing the decarboxylation of oxalyl-CoA to formyl-CoA. The gene encoding a novel oxalyl-CoA decarboxylase from Bifidobacterium lactis DSM 10140 (oxc) was identified and characterized. This strain, isolated from yogurt, showed the highest oxalate-degrading activity in a preliminary screening with 12 strains belonging to Bifidobacterium, an anaerobic intestinal bacterial group largely used in probiotic products. The oxc gene was isolated by probing a B. lactis genomic library with a probe obtained by amplification of the oxalyl-CoA decarboxylase gene from Oxalobacter formigenes, an anaerobic bacterium of the human intestinal microflora. The oxc DNA sequence analysis revealed an open reading frame of 1,773 bp encoding a deduced 590-amino-acid protein with a molecular mass of about 63 kDa. Analysis of amino acid sequence showed a significant homology (47%) with oxalyl-CoA decarboxylase of O. formigenes and a typical thiamine pyrophosphate-binding site that has been reported for several decarboxylase enzymes. Primer extension experiments with oxc performed by using RNA isolated from B. lactis identified the transcriptional start site 28 bp upstream of the ATG start codon, immediately adjacent to a presumed promoter region. The protein overexpressed in Escherichia coli cross-reacted with an anti-O. formigenes oxalyl-CoA decarboxylase antibody. Enzymatic activity, when evaluated by capillary electrophoresis analysis, demonstrated that the consumption substrate oxalyl-CoA was regulated by a product inhibition of the enzyme. These findings suggest a potential role for Bifidobacterium in the intestinal degradation of oxalate.
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