ArticleGenome biology2004
Large-scale exploration of growth inhibition caused by overexpression of genomic fragments in Saccharomyces cerevisiae.
Article in Genome biology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 45 citations in OpenAlex.
- Mutated Transcripts ofMolecular syndromology · 2023Article
- Engineering of Synthetic Transcriptional Switches in Yeast.Life (Basel, Switzerland) · 2022Review
- Peptide-tiling screens of cancer drivers reveal oncogenic protein domains and associated peptide inhibitors.Cell systems · 2021Article
- A Depletion of Stop Codons in lincRNA is Owing to Transfer of Selective Constraint from Coding Sequences.Molecular biology and evolution · 2020Article
- High-throughput identification of dominant negative polypeptides in yeast.Nature methods · 2019Article
- Autophosphorylation and Cross-Phosphorylation of Protein Kinases from the CrenarchaeonFrontiers in microbiology · 2017Article
- Quantitative nature of overexpression experiments.Molecular biology of the cell · 2015Article
- New genes from non-coding sequence: the role of de novo protein-coding genes in eukaryotic evolutionary innovation.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2015Review
- A tetO Toolkit To Alter Expression of Genes in Saccharomyces cerevisiae.ACS synthetic biology · 2015Article
- Aneuploid proliferation defects in yeast are not driven by copy number changes of a few dosage-sensitive genes.Genes & development · 2015Article
- Macrotene chromosomes provide insights to a new mechanism of high-order gene amplification in eukaryotes.Nature communications · 2015Article
- Expression of the bacterial type III effector DspA/E in Saccharomyces cerevisiae down-regulates the sphingolipid biosynthetic pathway leading to growth arrest.The Journal of biological chemistry · 2014Article
- Identification of dosage-sensitive genes in Saccharomyces cerevisiae using the genetic tug-of-war method.Genome research · 2013Article
- Proto-genes and de novo gene birth.Nature · 2012Article
- Expressing the Erwinia amylovora type III effector DspA/E in the yeast Saccharomyces cerevisiae strongly alters cellular trafficking.FEBS open bio · 2011Article
- Selection systems based on dominant-negative transcription factors for precise genetic engineering.Nucleic acids research · 2010Article
- Neurospora crassa mat A-2 and mat A-3 proteins weakly interact in the yeast two-hybrid system and affect yeast growth.Genetics and molecular biology · 2009Article
- An endoribonuclease functionally linked to perinuclear mRNP quality control associates with the nuclear pore complexes.PLoS biology · 2009Article
- Covert genetic selections to optimize phenotypes.PloS one · 2007Article
- A high-resolution map of transcription in the yeast genome.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
Corrections and comments
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Authors and funding
15 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have screened the genome of Saccharomyces cerevisiae for fragments that confer a growth-retardation phenotype when overexpressed in a multicopy plasmid with a tetracycline-regulatable (Tet-off) promoter. We selected 714 such fragments with a mean size of 700 base-pairs out of around 84,000 clones tested. These include 493 in-frame open reading frame fragments corresponding to 454 distinct genes (of which 91 are of unknown function), and 162 out-of-frame, antisense and intergenic genomic fragments, representing the largest collection of toxic inserts published so far in yeast.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.