ArticleMolecular biology of the cell2004
SMP-1, a member of a new family of small myristoylated proteins in kinetoplastid parasites, is targeted to the flagellum membrane in Leishmania.
Article in Molecular biology of the cell, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 59 papers.
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Who cites it
59 citing papers in PubMed, 104 citations in OpenAlex.
- A dual-aptazyme system for genetic complementation reveals stage-specific roles for themSphere · 2026Article
- The Intracellular Amastigote of Trypanosoma cruzi Maintains an Actively Beating Flagellum.mBio · 2023Article
- New Vistas in the Biology of the Flagellum-Pathogens (Basel, Switzerland) · 2022Review
- Proteomic Analysis ofPathogens (Basel, Switzerland) · 2021Article
- The single flagellum ofJournal of cell science · 2020Article
- Touching the Surface: Diverse Roles for the Flagellar Membrane in Kinetoplastid Parasites.Microbiology and molecular biology reviews : MMBR · 2020Review
- Isolation of Leishmania Promastigote Flagella.Methods in molecular biology (Clifton, N.J.) · 2020Article
- Expression and cellular localisation of Trypanosoma cruzi calpains.Memorias do Instituto Oswaldo Cruz · 2020Article
- Disruption ofBone research · 2020Article
- High-speed multifocal plane fluorescence microscopy for three-dimensional visualisation of beating flagella.Journal of cell science · 2019Article
- Lipid Modifications in Cilia Biology.Journal of clinical medicine · 2019Review
- Genetic dissection of a Leishmania flagellar proteome demonstrates requirement for directional motility in sand fly infections.PLoS pathogens · 2019Article
- Article
- Calpains of Leishmania braziliensis: genome analysis, differential expression, and functional analysis.Memorias do Instituto Oswaldo Cruz · 2019Article
- Dependency relationships between IFT-dependent flagellum elongation and cell morphogenesis inOpen biology · 2018Article
- Dynamic protein S-palmitoylation mediates parasite life cycle progression and diverse mechanisms of virulence.Critical reviews in biochemistry and molecular biology · 2017Review
- Tris DBA palladium is highly effective against growth and metastasis of pancreatic cancer in an orthotopic model.Oncotarget · 2016Article
- The N-myristoylome of Trypanosoma cruzi.Scientific reports · 2016Article
- Global Profiling and Inhibition of Protein Lipidation in Vector and Host Stages of the Sleeping Sickness ParasiteACS infectious diseases · 2016Article
- Flagellar pocket restructuring through the Leishmania life cycle involves a discrete flagellum attachment zone.Journal of cell science · 2016Article
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Authors and funding
10 authors at 1 institution in 1 country.
Funding
Abstract
The mechanisms by which proteins are targeted to the membrane of eukaryotic flagella and cilia are largely uncharacterized. We have identified a new family of small myristoylated proteins (SMPs) that are present in Leishmania spp and related trypanosomatid parasites. One of these proteins, termed SMP-1, is targeted to the Leishmania flagellum. SMP-1 is myristoylated and palmitoylated in vivo, and mutation of Gly-2 and Cys-3 residues showed that both fatty acids are required for flagellar localization. SMP-1 is associated with detergent-resistant membranes based on its recovery in the buoyant fraction after Triton X-100 extraction and sucrose density centrifugation and coextraction with the major surface glycolipids in Triton X-114. However, the flagellar localization of SMP-1 was not affected when sterol biosynthesis and the properties of detergent-resistant membranes were perturbed with ketoconazole. Remarkably, treatment of Leishmania with ketoconazole and myriocin (an inhibitor of sphingolipid biosynthesis) also had no affect on SMP-1 localization, despite causing the massive distension of the flagellum membrane and the partial or complete loss of internal axoneme and paraflagellar rod structures, respectively. These data suggest that flagellar membrane targeting of SMP-1 is not dependent on axonemal structures and that alterations in flagellar membrane lipid composition disrupt axoneme extension.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.