ArticleMolecular biology of the cell2004
Regulation of cell motility by tyrosine phosphorylated villin.
Article in Molecular biology of the cell, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 46 citations in OpenAlex.
- F-actin-bundling sites are conserved in proteins with villin-type headpiece domains.Molecular biology of the cell · 2020Article
- Villin-1 and Gelsolin Regulate Changes in Actin Dynamics That Affect Cell Survival Signaling Pathways and Intestinal Inflammation.Gastroenterology · 2018Article
- Development and characterization of 2-dimensional culture for buffalo intestinal cells.Cytotechnology · 2018Article
- Advillin acts upstream of phospholipase C ϵ1 in steroid-resistant nephrotic syndrome.The Journal of clinical investigation · 2017Article
- By moonlighting in the nucleus, villin regulates epithelial plasticity.Molecular biology of the cell · 2016Article
- Apical invasion of intestinal epithelial cells by Salmonella typhimurium requires villin to remodel the brush border actin cytoskeleton.Cell host & microbe · 2015Article
- Enterocyte loss of polarity and gut wound healing rely upon the F-actin-severing function of villin.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Regulation of intestinal epithelial cell cytoskeletal remodeling by cellular immunity following gut infection.Mucosal immunology · 2013Article
- Loss of villin immunoexpression in colorectal carcinoma is associated with poor differentiation and survival.ISRN gastroenterology · 2013Article
- Regulation of directional cell migration by membrane-induced actin bundling.Journal of cell science · 2013Article
- Actin reorganization as the molecular basis for the regulation of apoptosis in gastrointestinal epithelial cells.Cell death and differentiation · 2012Article
- Villin expression is frequently lost in poorly differentiated colon cancer.The American journal of pathology · 2012Article
- FAK phosphorylation at Tyr-925 regulates cross-talk between focal adhesion turnover and cell protrusion.Molecular biology of the cell · 2011Article
- Tumor lymphangiogenesis and metastasis to lymph nodes induced by cancer cell expression of podoplanin.The American journal of pathology · 2010Article
- Differential effects of lysophosphatidic acid and phosphatidylinositol 4,5-bisphosphate on actin dynamics by direct association with the actin-binding protein villin.The Journal of biological chemistry · 2009Article
- Galectin 1 proangiogenic and promigratory effects in the Hs683 oligodendroglioma model are partly mediated through the control of BEX2 expression.Neoplasia (New York, N.Y.) · 2009Article
- Potential molecular mechanism for c-Src kinase-mediated regulation of intestinal cell migration.The Journal of biological chemistry · 2008Article
- Regulation of cell structure and function by actin-binding proteins: villin's perspective.FEBS letters · 2008Review
- Autotaxin and lysophosphatidic acid stimulate intestinal cell motility by redistribution of the actin modifying protein villin to the developing lamellipodia.Experimental cell research · 2008Article
- Extracellular signal-regulated kinase/mitogen-activated protein kinase regulates actin organization and cell motility by phosphorylating the actin cross-linking protein EPLIN.Molecular and cellular biology · 2007Article
Corrections and comments
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Authors and funding
10 authors at 1 institution in 1 country.
Funding
Abstract
Temporal and spatial regulation of the actin cytoskeleton is vital for cell migration. Here, we show that an epithelial cell actin-binding protein, villin, plays a crucial role in this process. Overexpression of villin in doxycyline-regulated HeLa cells enhanced cell migration. Villin-induced cell migration was modestly augmented by growth factors. In contrast, tyrosine phosphorylation of villin and villin-induced cell migration was significantly inhibited by the src kinase inhibitor 4-amino-5-(4-chlorophenyl)-7-(t-butyl)pyrazolo[3,4-d]pyrimidine (PP2) as well as by overexpression of a dominant negative mutant of c-src. These data suggest that phosphorylation of villin by c-src is involved in the actin cytoskeleton remodeling necessary for cell migration. We have previously shown that villin is tyrosine phosphorylated at four major sites. To further investigate the role of tyrosine phosphorylated villin in cell migration, we used phosphorylation site mutants (tyrosine to phenylalanine or tyrosine to glutamic acid) in HeLa cells. We determined that tyrosine phosphorylation at residues 60, 81, and 256 of human villin played an essential role in cell migration as well as in the reorganization of the actin cytoskeleton. Collectively, these studies define how biophysical events such as cell migration are actuated by biochemical signaling pathways involving tyrosine phosphorylation of actin binding proteins, in this case villin.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.