ArticleJournal of bacteriology2004
Amino acid contacts between sigma 70 domain 4 and the transcription activators RhaS and RhaR.
Article in Journal of bacteriology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 45 citations in OpenAlex.
- Nucleotide-level characterization and improvement of l-arabinose- and l-rhamnose-inducible systems in E. coli using a high-throughput approach.Nucleic acids research · 2025Article
- Interactions between the AraC/XylS-like transcriptional activator InvF of Salmonella Typhimurium, the RNA polymerase alpha subunit and the chaperone SicA.Scientific reports · 2024Article
- Fructose-1-kinase has pleiotropic roles inbioRxiv : the preprint server for biology · 2023Article
- The l-rhamnose-dependent regulator RhaS and its target promoters fromFrontiers in microbiology · 2022Article
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- The transcriptional activator of the bfp operon in EPEC (PerA) interacts with the RNA polymerase alpha subunit.Scientific reports · 2021Article
- Article
- Mapping of protein-protein interactions of E. coli RNA polymerase with microfluidic mechanical trapping.PloS one · 2014Article
- Transcription activation by Escherichia coli Rob at class II promoters: protein-protein interactions between Rob's N-terminal domain and the σ(70) subunit of RNA polymerase.Journal of molecular biology · 2012Article
- Transcription activation by the siderophore sensor Btr is mediated by ligand-dependent stimulation of promoter clearance.Nucleic acids research · 2012Article
- A versatile element for gene addition in bacterial chromosomes.Nucleic acids research · 2012Article
- Different requirements for σ Region 4 in BvgA activation of the Bordetella pertussis promoters P(fim3) and P(fhaB).Journal of molecular biology · 2011Article
- H-NS binding and repression of the ctx promoter in Vibrio cholerae.Journal of bacteriology · 2011Article
- Promoter discrimination at class I MarA regulon promoters mediated by glutamic acid 89 of the MarA transcriptional activator of Escherichia coli.Journal of bacteriology · 2011Article
- Transcriptional control in the prereplicative phase of T4 development.Virology journal · 2010Review
- Protein-protein interactions between sigma(70) region 4 of RNA polymerase and Escherichia coli SoxS, a transcription activator that functions by the prerecruitment mechanism: evidence for "off-DNA" and "on-DNA" interactions.Journal of molecular biology · 2010Article
- ExsA recruits RNA polymerase to an extended -10 promoter by contacting region 4.2 of sigma-70.Journal of bacteriology · 2010Article
- The AraC/XylS family activator RhaS negatively autoregulates rhaSR expression by preventing cyclic AMP receptor protein activation.Journal of bacteriology · 2010Article
- A basic/hydrophobic cleft of the T4 activator MotA interacts with the C-terminus of E.coli sigma70 to activate middle gene transcription.Molecular microbiology · 2008Article
- TtsI regulates symbiotic genes in Rhizobium species NGR234 by binding to tts boxes.Molecular microbiology · 2008Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The RhaS and RhaR proteins are transcription activators that respond to the availability of L-rhamnose and activate transcription of the operons in the Escherichia coli L-rhamnose catabolic regulon. RhaR activates transcription of rhaSR, and RhaS activates transcription of the operon that encodes the L-rhamnose catabolic enzymes, rhaBAD, as well as the operon that encodes the L-rhamnose transport protein, rhaT. RhaS is 30% identical to RhaR at the amino acid level, and both are members of the AraC/XylS family of transcription activators. The RhaS and RhaR binding sites overlap the -35 hexamers of the promoters they regulate, suggesting they may contact the sigma70 subunit of RNA polymerase as part of their mechanisms of transcription activation. In support of this hypothesis, our lab previously identified an interaction between RhaS residue D241 and sigma70 residue R599. In the present study, we first identified two positively charged amino acids in sigma70, K593 and R599, and three negatively charged amino acids in RhaR, D276, E284, and D285, that were important for RhaR-mediated transcription activation of the rhaSR operon. Using a genetic loss-of-contact approach we have obtained evidence for a specific contact between RhaR D276 and sigma70 R599. Finally, previous results from our lab separately showed that RhaS D250A and sigma70 K593A were defective at the rhaBAD promoter. Our genetic loss-of-contact analysis of these residues indicates that they identify a second site of contact between RhaS and sigma70.
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