ArticleJournal of bacteriology2004
Denitrification genes regulate Brucella virulence in mice.
Article in Journal of bacteriology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 48 citations in OpenAlex.
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- The Retrospective on AtypicalMicroorganisms · 2022Review
- Uncovering the Hidden Credentials ofMicrobiology and molecular biology reviews : MMBR · 2021Review
- Biophysical and In-Silico Studies of Phytochemicals Targeting Chorismate Synthase from Drug-Resistant Moraxella Catarrhalis.The protein journal · 2020Article
- Survival of Brucella abortus S19 and other Brucella spp. in the presence of oxidative stress and within macrophages.Folia microbiologica · 2020Article
- Brucella abortus nitric oxide metabolite regulates inflammasome activation and IL-1β secretion in murine macrophages.European journal of immunology · 2019Article
- Origin and Impact of Nitric Oxide in Pseudomonas aeruginosa Biofilms.Journal of bacteriology · 2016Review
- Decreased in vivo virulence and altered gene expression by a Brucella melitensis light-sensing histidine kinase mutant.Pathogens and disease · 2015Article
- Article
- RegA, the regulator of the two-component system RegB/RegA of Brucella suis, is a controller of both oxidative respiration and denitrification required for chronic infection in mice.Infection and immunity · 2013Article
- Shift in ribonucleotide reductase gene expression in Pseudomonas aeruginosa during infection.Infection and immunity · 2011Article
- The Moraxella catarrhalis nitric oxide reductase is essential for nitric oxide detoxification.Journal of bacteriology · 2011Article
- Biochemical and genomic analysis of the denitrification pathway within the genus Neisseria.Microbiology (Reading, England) · 2009Article
- Brucella melitensis, B. neotomae and B. ovis elicit common and distinctive macrophage defense transcriptional responses.Experimental biology and medicine (Maywood, N.J.) · 2009Article
- Novel denitrifying bacterium Ochrobactrum anthropi YD50.2 tolerates high levels of reactive nitrogen oxides.Applied and environmental microbiology · 2009Article
- Genomic island 2 of Brucella melitensis is a major virulence determinant: functional analyses of genomic islands.Journal of bacteriology · 2008Article
- Role of large sequence polymorphisms (LSPs) in generating genomic diversity among clinical isolates of Mycobacterium tuberculosis and the utility of LSPs in phylogenetic analysis.Journal of clinical microbiology · 2007Article
- Identification of Pseudomonas aeruginosa genes involved in virulence and anaerobic growth.Infection and immunity · 2006Article
- Assessing the impact of denitrifier-produced nitric oxide on other bacteria.Applied and environmental microbiology · 2006Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Brucella is the causative agent of the zoonotic disease brucellosis, which is endemic in many parts of the world. Genome sequencing of B. suis and B. melitensis revealed that both are complete denitrifiers. To learn more about the role of denitrification in these animal pathogens, a study of the role of denitrification in the closely related B. neotomae was undertaken. In contrast to B. suis and B. melitensis, it was found that B. neotomae is a partial denitrifier that can reduce nitrate to nitrite but no further. Examination of the B. neotomae genome showed that a deletion in the denitrification gene cluster resulted in complete loss of nirV and the partial deletion of nirK and nnrA. Even though the nor operon is intact, a norC-lacZ promoter fusion was not expressed in B. neotomae. However, the norC-lacZ fusion was expressed in the related denitrifier Agrobacterium tumefaciens, suggesting that the lack of expression in B. neotomae is due to inactivation of NnrA. A narK-lacZ promoter fusion was found to exhibit nitrate-dependent expression consistent with the partial denitrifier phenotype. Complementation of the deleted region in B. neotomae by using nirK, nirV, and nnrA from B. melitensis restored the ability of B. neotomae to reduce nitrite. There was a significant difference in the death of IRF-1-/- mice when infected with B. neotomae containing nirK, nirV, and nnrA and those infected with wild-type B. neotomae. The wild-type strain killed all the infected mice, whereas most of the mice infected with B. neotomae containing nirK, nirV, and nnrA survived.
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Registered trials
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