ArticleJournal of bacteriology2004
Involvement of a plasmid-encoded type IV secretion system in the plant tissue watersoaking phenotype of Burkholderia cenocepacia.
Article in Journal of bacteriology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed.
- Genomic Insights into a New Burkholderia cenocepacia Sequence Type Linked to Cepacia Syndrome in Cystic Fibrosis.Current microbiology · 2026Article
- Secretion systems shaping host-pathogen interactions in Burkholderia species.Applied microbiology and biotechnology · 2026Review
- Quorum sensing in theApplied and environmental microbiology · 2026Review
- Pathogens without borders: a review on cross-kingdom transmission strategies and pathogenicity of plant and human pathogens.Archives of microbiology · 2026Review
- Combined Clinical, Epidemiological, and Genome-Based Analysis Identified a Nationwide Outbreak ofOpen forum infectious diseases · 2022Article
- Precise measurement of the fitness effects of spontaneous mutations by droplet digital PCR in Burkholderia cenocepacia.Genetics · 2021Article
- Complete genome dynamics of a dominant-lineage strain ofAccess microbiology · 2019Article
- Article
- Comparative Genomic Insights into Endofungal Lifestyles of Two Bacterial Endosymbionts, Mycoavidus cysteinexigens and Burkholderia rhizoxinica.Microbes and environments · 2018Article
- Genetic Determinants Associated WithFrontiers in microbiology · 2018Article
- Exploring bacterial interspecific interactions for discovery of novel antimicrobial compounds.Microbial biotechnology · 2017Article
- A Functional oriT in the Ptw Plasmid of Burkholderia cenocepacia Can Be Recognized by the R388 Relaxase TrwC.Frontiers in molecular biosciences · 2016Article
- Agrobacterium infection and plant defense-transformation success hangs by a thread.Frontiers in plant science · 2013Review
- Draft Genome Sequences of Burkholderia cenocepacia ET12 Lineage Strains K56-2 and BC7.Genome announcements · 2013Article
- Comparative genome analysis of Burkholderia phytofirmans PsJN reveals a wide spectrum of endophytic lifestyles based on interaction strategies with host plants.Frontiers in plant science · 2013Article
- Genome sequence of Pantoea sp. strain Sc 1, an opportunistic cotton pathogen.Journal of bacteriology · 2012Article
- Exploring the metabolic network of the epidemic pathogen Burkholderia cenocepacia J2315 via genome-scale reconstruction.BMC systems biology · 2011Article
- Evolution of an endofungal lifestyle: Deductions from the Burkholderia rhizoxinica genome.BMC genomics · 2011Article
- Burkholderia cenocepacia differential gene expression during host-pathogen interactions and adaptation to the host environment.Frontiers in cellular and infection microbiology · 2011Article
- Host evasion by Burkholderia cenocepacia.Frontiers in cellular and infection microbiology · 2011Review
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Burkholderia cenocepacia strain K56-2, a representative of the Burkholderia cepacia complex, is part of the epidemic and clinically problematic ET12 lineage. The strain produced plant tissue watersoaking (ptw) on onion tissue, which is a plant disease-associated trait. Using plasposon mutagenesis, mutants in the ptw phenotype were generated. The translated sequence of a disrupted gene (ptwD4) from a ptw-negative mutant showed homology to VirD4-like proteins. Analysis of the region proximal to the transfer gene homolog identified a gene cluster located on the 92-kb resident plasmid that showed homology to type IV secretion systems. The role of ptwD4, ptwC, ptwB4, and ptwB10 in the expression of ptw activity was determined by conducting site-directed mutagenesis. The ptw phenotype was not expressed by K56-2 derivatives with a disruption in ptwD4, ptwB4, or ptwB10 but was observed in a derivative with a disruption in ptwC. Complementation of ptw-negative K56-2 derivatives in trans resulted in complete restoration of the ptw phenotype. In addition, analysis of culture supernatants revealed that the putative ptw effector(s) was a secreted, heat-stable protein(s) that caused plasmolysis of plant protoplasts. A second chromosomally encoded type IV secretion system with complete homology to the VirB-VirD system was identified in K56-2. Site-directed mutagenesis of key secretory genes in the VirB-VirD system did not affect expression of the ptw phenotype. Our findings indicate that in strain K56-2, the plasmid-encoded Ptw type IV secretion system is responsible for the secretion of a plant cytotoxic protein(s).
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